6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系  

The Relationship Between the Effect of Exogenous 6-BA in Retarding Soybean Leaf Senescence and Plasma Membrane Protein Phosphorylation

作　　者：王宁宁[1] 王勇[1] 王淑芳[1] 朱亮基[1] 张韧 

机构地区：[1]南开大学生物化学与分子生物学系,天津300071 [2]wollongong大学生物系

出　　处：《植物生理学报（0257-4829)》1998年第3期305-308,共4页Acta Phytophysiologica Sinica

摘　　要：The plasma membrane fortion isolated from primp leaves of 14 d soybean shoot showed Protein kinase (PK) and protein phosphatase (PP) activities (Fig. 1 ), and these activities declined dramaically when the leaves underwent senescence-inducing treatment (Table 1 ). 6-BA pretreatment, which showed retarding effect on soybean leaf senescence, could sustain the activities of both PKs and PPs during the senescence-inducing treatment (Table 1 ). The results of autoradiogra phy suggested that the main PKs whose activities declined during leaf senescence were calcium-dependent Protein kinases (Fig. 3). The potent and specif ic inhibitor of Protein phosphatase 1 and 2A, okadric acid, could fasten leaf senescence. These results impied thatretaining the balance of plasma membrane protein phosphorylation/ dephosphorylation was the key action of exogenous 6-BA in retarding leat senescence.The plasma membrane fortion isolated from primp leaves of 14 d soybean shoot showed Protein kinase (PK) and protein phosphatase (PP) activities (Fig. 1 ), and these activities declined dramaically when the leaves underwent senescence-inducing treatment (Table 1 ). 6-BA pretreatment, which showed retarding effect on soybean leaf senescence, could sustain the activities of both PKs and PPs during the senescence-inducing treatment (Table 1 ). The results of autoradiogra phy suggested that the main PKs whose activities declined during leaf senescence were calcium-dependent Protein kinases (Fig. 3). The potent and specif ic inhibitor of Protein phosphatase 1 and 2A, okadric acid, could fasten leaf senescence. These results impied thatretaining the balance of plasma membrane protein phosphorylation/ dephosphorylation was the key action of exogenous 6-BA in retarding leat senescence.

关 键 词：大豆叶片 衰老 质膜蛋白磷酸化 脱磷酸化 

分 类 号：Q945[生物学—植物学] S565.101[农业科学—作物学]