雪松聚球藻金属硫蛋白的纯化及其性质的研究  

PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN FROM SYNECHOCOCCUS CEDRORUM

作　　者：夏建荣[1] 戴玲芬[2] 林惠民[2] 

机构地区：[1]汕头大学科学研究院能源与环境科学研究所,515063 [2]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》1998年第2期112-118,共7页Acta Hydrobiologica Sinica

基　　金：中国科学院水生生物研究所所长基金!950502

摘　　要：在雪松聚球藻的培养基中逐渐增加氯化镉的浓度以诱导藻细胞内金属硫蛋白（NT）的合成，经SephedexG－50、DEAE－cellulose（DE－52）和SephadexG－25柱层析，获得的MT没有亚型，经SDS－PAGE分析是高度均一的。每个蛋白分子约含5个Cd原子，10个巯基，分子量约为7．6KD，等电点pH4、5左右，半胱氨酸含量约占总氨基酸量的15．5％，还含有少量的芳香族氨基酸，MT的最大紫外吸收在250nm左右，在低pH下稳定性较差。The metallothionein (MT) is induced from synechococcus cedrorum growing in medium added gradually with cadmium chloride,and purified and characterized by means of Sephadex G-50. DEAE-cellulose (DE-52) and Sephadex G-25 colunm chromatography. The results show MT has no isoforms and is demonstrated to be homogenous by SDS-PAGE. Each molecule of MT contains 10 mercapto groups and combines with 5 cadmium atoms. Its molechlar weight is about 7.6KD,with isoelectric point being about pH4.5.Its cysteine resides account for 15.5% of all amino acid resides. The MT with a characteristic absorption maxium at 250nm is poorly stable with a large decrease of the absorption peak in low pH, and contains a little aromatic acid.

关 键 词：雪松聚球藻 金属硫蛋白 纯化 性质 

分 类 号：Q513.03[生物学—生物化学]