雄激素受体融合蛋白的表达及其多克隆抗体的制备与纯化  

Expression of Fusion Protein Containing Androgen Receptor Segment and Preparation of Polyclonal Antibodies to Androgen Receptor

作　　者：解芳[1] 平蓓芳[1] 张祖传[1] 张永莲[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第4期382-386,共5页

基　　金：国家自然科学基金

摘　　要：将雄激素受体（ＡＲ）的ｃＤＮＡ片段（１１０５～２２２４）克隆到表达质粒ｐＧＥＸ－３Ｘ内，在Ｅ．ｃｏｌｉ中表达，得到可溶性表达产物ＧＳＴ－ＡＲ。用该融合蛋白制得的兔多克隆抗体，经ＧＳＴ－Ｓｅｐｈａｒｏｓｅ－４Ｂ亲和吸附纯化后，表现出较好的抗ＡＲ的专一性。The cDNA segment (11052224) of the androgen receptor was cloned into the pGEX3X expression vector and expressed in E.coli. The soluble fusion proteins GSTAR were used to immunize rabbits to obtain polyclonal antibodies to androgen receptors. The antibodies were purified by GSTSepharose 4B and indicated the specificity to androgen receptors. The purified antibodies can be used in study the function of AR.

关 键 词：雄激素受体 融合蛋白 多克隆抗体 亲合层析 

分 类 号：Q78[生物学—分子生物学]