稻瘟病菌致病突变体的REMI诱变与鉴定  

REMI Mutagenesis and Identification of Pathogenic Mutants in Magnaporthe grisea

作　　者：刘树俊[1] 魏荣宣[1] 有江力[2] 山口勇 

机构地区：[1]中国科学院遗传所植物细胞与染色体工程国家重点实验室 [2]日本理化学研究所微生物制御研究室

出　　处：《生物工程学报》1998年第3期254-258,共5页Chinese Journal of Biotechnology

摘　　要：以水稻“爱知旭”（Ａｉｃｈｉａｓｈａｈｉ）为寄主，将带选择标记的质粒ｐＣＳＮ４３和ｐＢＦ１０１为外源ＤＮＡ，利用限制酶诱导整合（ＲＥＭＩ）这种新方法转化稻瘟病菌原生质体，从筛选到的数百个转化体中分离出３个与致病能力密切相关的突变体Ｒ２Ｈ６５、Ｒ２Ｈ６９和Ｒ２Ｂ１５６５。其中，Ｒ２Ｈ６５和Ｒ２Ｈ６９只产生畸形分生孢子，分生孢子的发育和附着胞的形成以及黑色素的合成均受到极大影响，致病性测试证明完全丧失致病能力；Ｒ２Ｂ１５６５的许多表型与野生型的相似，但致病能力却大大降低。Rice-Aichi ashahi was used as host and plasmid pBF101 or pCSN43 as foreign DNA f ragment,REMI mutagenesis was applied to tag pathogenic sequence in rice blast fungus M.grisea ,three mutants R2H65,R2H69 and R2B1565,related to pathogenicity,were recovered from several hundreds of transformants.R2H69 and R2H65 produced merely strange conidia which could not form appressorium or yield abnormal one,pathogenicity test further confirmed that they were unable to cause disease.R2B1565 owned the same phenotypes as those of wild type except that the ability of causing disease was much reduced.

关 键 词：致病性 稻瘟病菌 限制酶诱导整合 杀稻瘟菌素 

分 类 号：Q933[生物学—微生物学] S435.111.4[农业科学—农业昆虫与害虫防治]