不同纲动物间的细胞核移植——将小鼠细胞核移到泥鳅细胞质中  

Nuclear Transplantation Between Animals of Different Classes__the Combination of the Nucleus of Mouse and the Cytoplasm of Loach

作　　者：李书鸿[1] 毛钟荣 韩文[1] 陈惠萍[1] 严绍颐[1] 夏仕铃[2] 戴世行 

机构地区：[1]中国科学院发育生物学研究所 [2]中国水产科学院珠江水产研究所 [3]四川省水产学校

出　　处：《生物工程学报》1998年第3期345-347,共3页Chinese Journal of Biotechnology

基　　金：国家"七五"攻关基金

摘　　要：我们以前的细胞核移植工作［１，２］表明：移核卵早期胚胎发育模式（主要指卵裂速度及卵裂模式）与受体卵相同，而与供体核种类无关；供体与受体之间亲缘关系的远近及染色体数目的差异程度，对移核卵囊胚以后的发育有重要影响，但对不同组合间细胞核移植所得囊胚比例影响...The nucleus of a blastula cell from mouse(mus musculus albula,class mammalia,2N=40) was transplanted into an enucleated egg of loach (Paramisgurnus dabryanus,class pisces,2N=48). Of 79 nucleo cytoplasmic hybrid (NCH) eggs,20(25.3%)developed to blastula,but no gastrula was obtained.Developmental incompatibility between donor nucleus and recipient cytoplasm existed,which was expressed by the formation of multispindles.The multispindle was proposed to result in chromosome exclusion in the NCH egg.Such chromosome exclusion might be an important reason for the abnormal development of NCH egg and the cessation of the further development of NCH blastula in this mouse donor and loach recipient combination.

关 键 词：细胞核移植 不同纲动物间 小鼠 泥鳅 

分 类 号：Q78[生物学—分子生物学]