用多孔微载体大规模培养rCHO细胞  

Large scale Cultivation of rCHO Cells Using Porous Microcarriers

作　　者：胡显文[1] 肖成祖[1] 李文青[1] 黄子才[1] 张正光[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《生物工程学报》1998年第3期348-351,共4页Chinese Journal of Biotechnology

基　　金："863"计划资助项目

摘　　要：多孔微载体是近年来发展起来的一种用于大规模高密度培养动物细胞的支持物，具有许多优点，如：容易固定细胞，适合于贴壁细胞和悬浮细胞的固定化连续灌流培养；细胞生长在载体内部，增加了细胞固定化的稳定性，可降低血清用量，适合长期培养；能保护细胞免受机械损伤，增...Large scale culture of recombinant CHO cells was carried out with cytopore cellulose porous microcarriers in a 20L Biostat UC20 stirred tank reactor,which equipped with silicone tubing bubble free oxygenator.During 16 days semi continuous culture,the cell density reached 6.6×10 6/ml,and maximal pro UK activity was over 8 000IU/ml,and 55L supernatant contained 2.5g pro UK was harvested. The rCHO cells had been continuously cultivated for 28 days ,after 16 days culture,the cell density increased up to 1.33×10 7/ml,the maximal pro UK activity was 7335 IU/ml,204L supernatant was harvested,the pro UK production was 7.05g.Cells can move from seed microcarriers occupied by cells into vacant microcarriers spontaneously and continue to proliferate until all the microcarriers contained rCHO cells.It shows that the porous microcarriers is very useful and convenient for large scale cell culture.

关 键 词：RCHO细胞 培养 多孔微载体 

分 类 号：Q952[生物学—动物学]