穹窿海马伞损伤对前脑NOS阳性神经元的影响  

TRANSECTION OF RAT FIMBRIA-FORNIX INDUCES THE CHANGE OF AXOTOMIZED SEPTAL NEURONS IMMUNO-POSITIVE FOR NITRIC OXIDE SYNTHASE

作　　者：龙大宏[1] 王富强[1] 杨丹迪[1] 许孟杰[1] 李沃棠[1] 

机构地区：[1]广州医学院人体解剖学教研室,广州510182

出　　处：《神经解剖学杂志》1998年第2期183-186,共4页Chinese Journal of Neuroanatomy

摘　　要：本研究用NADPH－d组织化学方法，对成年大鼠一侧穹窿海马个切断后前脑NOS阳性神经元的变化进行了计量学分析。结果表明：损伤1周后，损伤侧内侧隔核和斜角带垂直支的NOS阳性神经元数分别减少到71．79％和85．86％，细胞面积缩小到86．34％和92．71％，细胞灰度值增大到111．86％和120．37％；损伤后2周，损伤侧内侧隔核和斜角带垂直支的NOS阳性神经元数量持续下降，分别减少到63．95％和73．56％，细胞面积缩小到61．78％和89．77％，灰度值增大到116．78％和128．96％；损伤后3、4周，损伤侧两核的NOS阳性神经元的变化和第2周时类似，数值保持在较稳定的水平。提示受损的前脑NOS阳性神经元发生退变。The fimbria-fornix fiber tract was unilaterlly transected in adult rats by a stereotaxic knife cut. NADPH-diaphorase histochemistry was used to assess quantitatively the change of nitric oxide synthase (NOS)-positive neurons in the forebrain at 1,2, 3,4 weeks after the operation. The number of neuronal loss was 28. 21% of NOS-labelled cells in the medial septal nucleus (MS) and 14. 14 % of NOS-labelled cells in the vertical branch of diagonal band (VDB) on lesion side by 7 days, the average size of the cell in MS was decreased by 13. 66% and 7. 29% of the cells in VDB, but the intensity of the reaction was increased by 11.86% of the cells in MS and 20. 37 % of the cells in VDB. Between 2, 3 and 4 week, the number of NOS-labelled cells was reduced by 36. 05% in MS and 26. 44% in VDB, the average area of NOS-positive cell was decreased by 38. 21 % in MS and 10. 33% in VDB. The intensity of the reaction was decreased by 16. 78% in MS and 28. 96% in VDB. On the contralateral side, the NOSlabelled neurons did not differ from those observed in untreated rats. The results revealed that the axotomized septal NOS-positive neurons presented rapid retrograde neuronal degeneration.

关 键 词：NOS 穹窿海马伞切断 内侧隔核 斜角带核 大鼠 

分 类 号：Q954.671[生物学—动物学] Q959.837