猪带绦虫腺苷酸激酶基因及蛋白结构特性分析  

Prediction and analysis for structure and function of adenylate kinase gene from Taenia solium by bioinformatics

作　　者：戴佳琳[1] 黄江[1] 廖兴江[1] 周灵贵[1] 申萍香[1] 刘玉江[1] 郎书源[2] 

机构地区：[1]贵阳医学院多媒体形态学实验室,贵阳550004 [2]贵阳医学院寄生虫学教研室

出　　处：《中国公共卫生》2009年第10期1214-1215,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30760227);贵州省科技攻关项目(2008-3060)

摘　　要：目的分析和预测猪带绦虫腺苷酸激酶（ADK）基因及其编码的蛋白结构和特性。方法利用各种在线生物信息网站及其它分析软件包,分析从猪带绦虫成虫cDNA质粒文库中识别的腺苷酸激酶基因的结构和编码区序列,分析、预测编码的蛋白质的理化特性、抗原表位、翻译后的修饰、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。结果该基因全长864 bp,编码区为110－758,编码215个氨基酸,为全长基因。理论分子量、等电点分别为23771.4 Da和6.86;没有跨膜区;三维结构建模图显示其线性表位的部位。结论应用生物信息方法从猪带绦虫成虫cDNA文库中筛选出了腺苷酸激酶基因的cDNA全长序列并预测得到其结构与功能方面的信息。Objective To analyze and predict the structure and characteristics of adenylate kinase gene from Taenia solium by bioinforrnatics for its biological function and application research. Methods With analyzing tools of bioinformatics in webs site and bioinformatics software package, adenylate kinase was recognized from the Taenia solium fulllength eDNA plasmid libratory and its gene structure and coding region sequence was analyzed and its physical-chemical characteristics,topological structure and 3 D structure were predicted. Results The full gene is 864 bp in length and its coding region is 110 -758, coding 215 amino acids. It is a complete full-length gene comparing with the homologues in GenBank. The protein has no transmembrane region. The molecular weight of adenylate kinase gene is predicted to be 23771.4Da. The proein contains no transmembrane region and subcellular location sequecce. The predicted amino acid sequence shows 66% identity and 84% similarity with that of ADK form Schistosoma japonicum. Conclusion The eDNA sequence encoded adenylate kinase gene was screened from eDNA library of adult Taenia solium by bioinformatical method. The gene and protein structure and characteristics of Taenia solium adenylate kinase were obtained.

关 键 词：猪带绦虫 腺苷酸激酶 CDNA 生物信息学 

分 类 号：R383.32[医药卫生—医学寄生虫学]