保定蝗区土壤绿僵菌对飞蝗高毒力菌株的筛选  

Screening for high virulence strains of Metarhizium anisopliae against Locusta migratoria manilensis from Baoding

作　　者：田甜[1] 李瑞军[1] 陆秀君[1] 张龙[2] 董建臻[1] 张振波 张怡[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,保定071001 [2]中国农业大学农业部生物防治重点开放实验室,北京100193 [3]河北省植保植检站,石家庄050011

出　　处：《植物保护》2009年第5期65-69,共5页Plant Protection

基　　金：“十一五”国家科技支撑计划(2005BA529A01);河北农业大学博士基金;河北农业大学科学发展基金

摘　　要：对由保定蝗区土壤中分离到的金龟子绿僵菌中毒力较好的10个菌株，测定了对东亚飞蝗3龄蝗蝻的僵虫率，并选出3株发病效果好的菌株M6—448、M5—110、M7—355，再采用点滴接种法对1～5龄蝗蝻及成虫进行了毒力测定。结果表明，不同菌株对飞蝗的毒力与菌株种类和蝗蝻龄期有密切关系，3株菌株对3龄蝗蝻处理5d的LD50分别为7．33×10^3、5．90×10^3、2．69×10^4个／mL，LT50分别为：3．70、5．86、7．50d；对成虫的LD50分别为5．49×10^5、5．09×10^5、2．28×10^6个／mL，LT50分别为8．03、8．07、8．57d。以毒力较高的M6—448菌株进行田间罩笼试验，10d对3龄蝗蝻校正防治效果可以达到93．6％，与2000倍4．5％高效氯氰菊酯乳油结果96．2％相近，具有进一步研究的价值。The high virulence strain of Metarhizium anisopliae isolated from Baoding was screened against the 3rd instar nymph and adults of Locusta migratoria manilensis （Meyen） by bioassay. The virulence of M6-448, M5-110 and M7-355 chosen in primary experiments were higher than the other isolates. The LDs0 of M6-448, M5-110 and M7-355 against the 3rd instar nymph and adults was 7.33N103 , 5.90×10^3 , 2.69×10^4 conidia/mL and 5.4×10^5 , 5.09×10^6 and 2. 28×10^6 eonidia/mL, respectively. The LT50 was 3. 70, 5. 86, 7. 50 d and 8. 03, 8. 07 d and 8. 57 d, respectively. These results demonstrated that M6-448 was most optimal among these strains. 93. 6% of the 3rd instar nymphae, treated by M6 448, was lethal in field experiments in 10 d, which was similar to chemical pesti- cide （beta-cypermethrin） （96.2%）. These results indicated that M6-448 isolate may be used as a potential wild strain against L. migratoria manilensis in practice in the future.

关 键 词：保定 金龟子绿僵菌 东亚飞蝗 生物测定 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]