精浆弹性硬蛋白酶与精液主要参数及精子功能相关性分析  

The relationship analysis of seminal plasma elastase with semen major parameters and sperm function

作　　者：王贺[1] 邹德学[1] 李世勤[1] 

机构地区：[1]北京大学深圳医院检验科,深圳518036

出　　处：《中国男科学杂志》2009年第9期55-57,62,共4页Chinese Journal of Andrology

摘　　要：目的探讨精浆弹性硬蛋白酶与精液主要参数及精子功能指标的关系。方法按照WHO《人类精液及精子-宫颈粘液相互作用实验室检验手册》要求,对170例不孕夫妇中的男性患者进行精液常规检测及精子功能分析,根据精浆弹性硬蛋白酶的检测结果将所有患者分为A、B、C3组。A组为精浆弹性硬蛋白酶含量>1000ng/ml;B组为精浆弹性硬蛋白酶含量290～1000 ng/ml;C组为精浆弹性硬蛋白酶含量<290ng/ml。通过比较三组的精液主要参数及精子功能指标来分析精浆弹性硬蛋白酶与男性不育各因素的相关性。结果 170例患者中A组56例(32.9%)、B组38例(22.4%)、C组76例(44.7%)。与C组相比A组在精子活率、活力、头部畸形、顶体酶活性指标方面均显著低下(P<0.01),而B组与C组相比上述指标差异无统计学意义(P>0.05),各组间精子密度及DNA完整性的差异无统计学意义(P>0.05)。结论精浆弹性硬蛋白酶与精液质量密切相关,精浆弹性硬蛋白酶作为男性生殖道感染的指标,在评估男性生育力、男性不育症的诊断和治疗,以及精子功能等方面有着重要的临床意义。Objective To investigate the relationship of the seminal plasma elastase with semen major parameters and sperm function. Methods The semen major parameters and sperm function of 170 infertile men were measured and analyzed according to WHO laboratory handbook on human semen. According to the concentration of seminal plasma elastase in semen, all patients were divided into group A（seminal plasma elastase 〉1 000ng/ml）,group B（seminal plasma elastase 290-1 000 ng/ml） and group c（seminal plasma elastase 〈290ng/ml）. The relationship between the seminal plasma elastase and the factors of infertile men were investigated by comparative analysis of the results of the semen major parameters and sperm function from the three groups. Results Among 170 male infertilities, 56 cases of them in group A （32.9%）, 38 cases in group B （22.4%）; 76 cases in group C （44.7%）. Sperm motility, a＋b motility, abnormal morphological rate, the activity of acrosin in the group A were all significantly lower than that in group C （P〈0.01）. But there was no difference in the above indexes between group C and group B （P〉0.05）. There was no difference in sperm concentration and DNA integrality among three groups （P〉0.05）. Conclusion The results suggested that seminal plasma elastase was closely associated with semen quality, and might be a potential index for diagnosis and treatment of male infertility and evaluation of sperm function, et al.

关 键 词：精浆 弹性硬蛋白酶 生殖道感染 不育 

分 类 号：R698.2[医药卫生—泌尿科学]