异种(猪)无细胞真皮基质的制备及体外生物相容性  

Preparation and in vitro biocompatibility of xenogenic (porcine) acellular dermal matrix

作　　者：马绍英[1] 李宝明[1] 董丽[1] 王旭昇[1] 李宝兴[1] 赵亚平[1] 康悦[1] 

机构地区：[1]中国辐射防护研究院生物材料与制药技术研究所,山西省太原市030006

出　　处：《中国组织工程研究与临床康复》2009年第38期7424-7428,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：山西省自然科学基金项目资助(2008011079-4)~~

摘　　要：背景:人同种无细胞真皮基质作为一种永久性真皮支架,已成功应用于烧伤创面修复及美容医学等领域,但由于来源有限,限制了其应用。目的:研制异种(猪)无细胞真皮基质,并对其体外生物相容性进行评价。设计、时间及地点:体外细胞学对比观察实验,于2007-08/2008-06在中国辐射防护研究院生物材料与制药技术研究所实验室完成。材料:实验猪由中国辐射防护研究院实验动物中心提供;人成纤维细胞来自武警山西总队医院健康儿童包皮环切术切除的包皮组织。方法:无菌条件下获取健康小白猪猪皮,用高渗盐溶液-去污剂、胰酶消化及超声清洗的方法,制备猪无细胞真皮基质。体外培养人成纤维细胞,用猪无细胞真皮基质浸提液法及人成纤维细胞和猪无细胞真皮基质直接贴附法,评价猪无细胞真皮基质体外生物相容性。主要观察指标:①猪无细胞真皮基质的组织学形态。②猪无细胞真皮基质的体外生物相容性。结果:制备的猪无细胞真皮基质,完全去除了表皮和真皮中的细胞成分,保留了胶原基质。猪无细胞真皮基质浸提液对人成纤维细胞增殖无明显影响。人成纤维细胞可以在猪无细胞真皮基质上贴附、增殖。结论:此种方法制备的无细胞真皮基质完全去除了表皮和真皮中的细胞成分,有较好的体外生物相容性。BACKGROUND： Human allogenic acellular dermal matrix, as a kind of permanent dermal scaffold, has widely used in the fields of burn wound reparation and aesthetic medicine. However, it is limited due to insufficient resources. OBJECTIVE： To prepare porcine acellular dermal matrix （PADM） dermal matrix, in addition, to estimate its in vitro biocompatibility. DESIGN, TIME AND SETTING： An in vitro cytology contrast experiment. The Experiment was performed at the taboratory of Biomaterials and Pharmacy Technology Institute, China Institute for Radiation Protection between August 2007 and June 2008. MATERIALS： The experiment pigs were supplied by experimental animal center of China Institute for Radiation Protection. Human fibroblasts were obtained from prepuce tissues of healthy children who underwent circumcision at the Shanxi Provincial General Hospital, Chinese People＇s Armed Police Forces. METHODS： The PADM was prepared from porcine skin by removing epidermis with a hypertonic salt solution and excluding cellular components in dermis with sodium dodecyl sulfate, and trypsin in hypersonic conditions. Human fibroblasts were cultured in vitro, and the biocompatibility of PADM was estimated with MTT method and contact method. MAIN OUTCOME MEASURES： ①Histological morphology of PADM. （2）In vitro biocompatibility of PADM. RESULTS： The prepared PADM was a kind of matrix with normal structure and organization of collagen without epidermis and any cellular components in the dermis. The extraction of the porcine acellular dermal matrix had no effect on proliferation of the cultured human fibroblast. The cultured human fibroblasts could attach and proliferate on PADM. CONCLUSION： The PADM effectively removed epidermis and all cellular components with excellent biocompatibility can be obtained by this preparation method.

关 键 词：猪无细胞真皮基质 人成纤维细胞:生物相容性 

分 类 号：R318[医药卫生—生物医学工程]