组织工程小血管支架应用于异种血管移植的可行性  

Feasibility of tissue engineered small vessel scaffolds applying to blood vessel transplantation

作　　者：陈华勇[1] 柏树令[2] 崔春兰[1] 

机构地区：[1]延边大学基础医学院解剖学与组织胚胎学教研室,吉林省延吉市133000 [2]中国医科大学组织工程教研部,辽宁省沈阳市110001

出　　处：《中国组织工程研究与临床康复》2009年第38期7429-7432,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：吉林省教委资助项目(01002)~~

摘　　要：背景:具有α半乳糖基末端的糖蛋白是引起超急性排斥反应的主要异种抗原。目的:观察脱细胞小血管支架与Wistar大鼠和日本大耳白兔两种种属实验动物血管壁内皮细胞和平滑肌细胞内α半乳糖基的分布特点,探讨经脱细胞处理后的小血管支架应用于异种血管移植的可行性。设计、时间及地点:观察对比实验,于2003-03/2004-12在中国医科大学解剖学教研室完成。材料:采集Wistar大鼠正常尾动脉30条,其中选择15条进行脱细胞处理得小血管支架(小血管支架组),剩余15条未进行脱细胞处理(尾动脉组);采集日本大耳白兔耳背面中央动脉,左右耳各1条,共取15条(中央动脉组)。方法:采用亲和组织化学法,将16mg/L植物凝集素加入组织片作DAB显色后,利用MetaMorth/C5050/BX41显微图像分析系统,检测α半乳糖基阳性反应产物。主要观察指标:光镜下血管壁细胞显色变化;α半乳糖基阳性反应产物的吸光度值。结果:中央动脉组α半乳糖基的表达主要集中于内皮细胞膜和细胞核;尾动脉组内皮细胞膜α半乳糖基表达呈极强阳性;小血管支架组呈弱阳性或阴性表达。3组血管细胞α半乳糖基表达的吸光度值中,尾动脉组内膜低于中央动脉组(P<0.001);小血管支架组内膜低于中央动脉组、尾动脉组(P<0.001);小血管支架组中膜低于中央动脉组、尾动脉组(P<0.001)。结论:①Wistar大鼠尾动脉内皮细胞膜异种抗原性比日本大耳白兔中央动脉的抗原性强。②Wistar大鼠脱细胞的尾动脉支架可作为异种组织工程血管材料应用于异种血管移植。BACKGROUND： The glycoprotein which ends of a-galactosyl residues （α-Gal） is the major heterogenic antigen for hyperacute rejection. OBJECTIVE： To observe the distribution characteristic of the small tissue engineered vessel scaffold, and α-Gal in endothelial cells and smooth muscle cells of Wistar rats and Japanese white rabbits, in addition, to discuss the feasibility of applying acellular tissue vessel scaffold to heterogeneous blood vessel transplantation. DESIGN, TIME AND SETTING： The contrast observation was conducted at the Department of Human Anatomy of China Medical University between March 2003 and December 2004. MATERIALS： Totally 30 caudal arteries of Wistar rats were collected. Fifteen of which were prepared for small vessel scaffold （small vessel scaffold group）, the remained 15 served as caudal artery group. Additionally, 15 central arteries were obtained from each ear of Japanese white rabbits （central artery group）. METHODS： Totally 16 mg/L Bandeiraea Simplicifolia I Isolectin B4 （BSI-B4） was added for DAB staining with Affinity histochemistry method. Then MetaMorth/C5050/BX41 microscopic image analysis system was used to detect the positive reaction product of α-Gal. MAIN OUTCOME MEASURES： Color changes of vascular wall were observed under light microscope; the optical density of the positive reaction product of α-Gal was measured. RESULTS： The expression of α-Gal was mainly located in the cell membrane, as well as cell nucleus of endothelial cells in the central artery group. The expression of α-Gal of endothelial cell was strong positive in the caudal artery group, which was weak or negative expressed in the small vessel scaffold group. The optical density of α-Gal expression was lowest in the tunica intima of small vessel scaffold group, which was less in the central artery group than the caudal artery group （P 〈 0.001）. The ct-Gal expression in the tunica media of small vessel scaffold group was less than the central artery and the caudal artery grou

关 键 词：异种 血管 α半乳糖基 

分 类 号：R318[医药卫生—生物医学工程]