酸性成纤维细胞因子/部分脱蛋白骨修复兔早期股骨头缺血性坏死的血管化作用(英文)  

Vascularization of acidic fibroblast growth factor/partially deproteinised bone in repair of early-stage avascular necrosis of the femoral head in rabbits

作　　者：朱肖奇[1,2] 郭浩[3] 

机构地区：[1]解放军兰州军区总院博士后工作站,甘肃省兰州市730050 [2]南方医科大学附属深圳宝安医院骨科,广东省深圳市518101 [3]南华大学,湖南省衡阳市421001

出　　处：《中国组织工程研究与临床康复》2009年第38期7469-7473,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：a Grant from Shenzhen Bureau of Science and Technology,No.200702043~~

摘　　要：背景:酸性成纤维细胞因子具有较好的促进血管形成作用以及成骨作用,能否有效地促进早期股骨头缺血性坏死动物模型的血管化有待研究。目的:探讨酸性成纤维细胞因子复合部分脱蛋白骨对兔股骨头缺血性坏死修复的血管化作用。设计、时间及地点:随机对照动物实验,于2008-01/2009-01在南华大学生命科学院完成。材料:取健康成年新西兰大白兔肋骨,经脱脂、脱蛋白、部分脱钙和冻干等一系列的物理、化学方法处理后制备部分脱蛋白骨。将酸性成纤维细胞生长因子用无菌蒸馏水稀释后加入部分脱蛋白骨粒,得到复合人工骨。方法:健康成年新西兰大白兔24只,双侧股骨颈交界处开窗,挖除股骨头内约50%松质骨,加用体积分数为95%乙醇灌注30min,建立双侧股骨头坏死骨缺损模型。按随机数字表法,随机分为空白组,单纯部分脱蛋白骨组,复合人工骨组。分别于相应股骨头骨缺损区植入部分脱蛋白骨、复合人工骨,空白组不植入材料。主要观察指标:分别于术后2,4,8周取材,制备墨汁灌注标本,进行微血管计数以及血管面积分析。结果:2,4,8周时血管计数和血管面积显示,空白组血管数和血管面积最少,单纯部分脱蛋白骨组次之,复合人工骨组最多。复合人工骨组与空白组和单纯部分脱蛋白骨组比较,差异有显著性意义(P<0.05)。结论:酸性成纤维细胞生长因子与部分脱蛋白骨复合构建组织工程化人工骨具有较好的促进血管生成作用,对兔早期股骨头缺血坏死具有较好的修复作用。BACKGROUND： Acidic fibroblast growth factor （aFGF） possesses good effects on vascularization and osteogenesis. But whether aFGF can promote the vascularization in animals with early-stage avascular necrosis of the femoral head （ANFH） remains unclear. OBJECTIVE： To investigate the vascularization of aFGF composited by partially deproteinized bone （PDPB） in repair of early-stage ANFH. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed in the College of Life Science, Nanhua University between January 2008 and January 2009. MATERIALS： Ribs from healthy, adult, New Zealand rabbits were prepared into PDPB by a series of physico-chemical methods including degreasing, deproteinization, partial decalcification and freeze drying, aFGF diluted with sterile distilled water was composited by PDPB particles to prepare artificial composite bone. METHODS： A bone window was made at the juncture of femoral head and femoral neck bilaterally in 24 healthy, adult, New Zealand rabbits. Rabbit models of bilateral ANFH were established by removing approximately 50% of cancellous bone and perfusion with 95% ethanol. Successful bilateral ANFH models were randomly divided into a blank group, a simple PDPB group, and an artificial composite bone group. PDPB and artificial composite bone were implanted into the PDPB and artificial composite bone group accordingly. The blank group did not receive any implantation. MAIN OUTCOME MEASURES： At 2, 4, and 8 weeks after surgery, ink-injected specimens were prepared for microvessel count and microvessel area analysis. RESULTS： Microvessel number and microvessel area were least in the blank group, followed by simple PDPB group, and lastly the artificial composite bone group. There was significant difference in microvessel number and microvessel area between artificial composite bone group and blank, simple PDPB groups （P 〈 0.05）. CONCLUSION： Tissue-engineered artificial bone composited by aFGF and PDPB promotes vascularization in repai

关 键 词：股骨头缺血性坏死 酸性成纤维细胞因子 兔 部分脱蛋白骨 血管化 

分 类 号：R318[医药卫生—生物医学工程]