钛合金种植体表面制备的生物活性膜  

Preparation of titanium implant surface bioactive membrane

作　　者：张晓光[1] 张扬[1] 

机构地区：[1]中国医科大学口腔医学院正畸科,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2009年第38期7489-7492,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：辽宁省自然科学基金项目(20082088)~~

摘　　要：背景:传统的仿生方法通常采用常规模拟体液和1.5倍模拟体液,其缺点就在于涂层生长过于缓慢,生长周期长达数周甚至几个月。目的:探讨3倍模拟体液诱导Ti-6Al-4V合金表面快速形成类羟基磷灰石涂层的能力。设计、时间及地点:体外生物学分析。实验于2008-11/2009-03在中国科学院金属研究所完成。材料:Ti-6Al-4V合金片由陕西省宝鸡市博达金属材料有限公司提供。方法:将经过预处理的Ti-6A1-4V合金浸泡在37℃的3倍模拟体液中,为保持浸泡液成分的恒定,溶液每24h更换1次,共浸泡3d。主要观察指标:用扫描电镜、X射线衍射仪和红外光谱仪分析涂层的形貌、相及元素组成。结果:扫描电镜观察样品浸泡1d时,表面被沉积膜覆盖,局部区域有较大颗粒状的晶体出现。浸泡3d时,表面完全被沉积膜覆盖,且有大量颗粒状或鳞片状晶体出现,晶体沉积以某一区域为核心,持续生长。生成物主要由Ca,P,O,C等元素组成,但是没有检测到Al,V等元素的存在。X射线衍射仪和红外光谱仪分析浸泡3d时Ti-6A1-4V合金表面制备的生物活性膜主要成分为羟基磷灰石。结论:使用3倍模拟体液可以快速诱导Ti-6Al-4V合金表面类羟基磷灰石的沉积,显著缩短涂层形成的时间。BACKGROUND： The coating induced by conventional simulated body fluid （SBF） and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE： To evaluate Ti-6A1-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING： In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS： Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS： The pretreated Ti-6A1-4V alloy was immersed in 37 ℃ 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES： The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS： The scanning electron microscopy exhibited that the surface of Ti-6A1-4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION： The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

关 键 词：TI-6AL-4V合金 高浓度模拟体液 仿生法 类羟基磷灰石 

分 类 号：R318[医药卫生—生物医学工程]