芳香/脂肪共聚酯生物材料与大鼠骨髓间充质干细胞的相容性实验  

Biocompatibility between aromatic/aliphatic copolyesters and bone marrow mesenchymal stem cells in rats

作　　者：谢安[1] 付建华[2] 陈义旺[3] 罗琳[2] 谈利承[3] 汪泱[1] 

机构地区：[1]南昌大学第一附属医院泌尿外科研究所,江西省南昌市330006 [2]南昌大学第一附属医院整形外科,江西省南昌市330006 [3]南昌大学材料科学与工程学院,江西省南昌市330031

出　　处：《中国组织工程研究与临床康复》2009年第38期7517-7522,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：南昌大学创新团队建设资助项目~~

摘　　要：背景:芳香族聚酯生物降解性很差,不能单独作为降解材料使用,南昌大学材料科学与工程学院通过对苯二甲酰氯、双酚A、己二醇和低聚乳酸通过熔融共缩聚和酯交换的方法合成出一种新型生物可降解材料——芳香/脂肪共聚酯,已申请专利。目的:观察芳香/脂肪共聚酯生物材料与大鼠骨髓间充质干细胞的相容性。设计、时间及地点:细胞-材料学体外实验,于2006-04/2007-04在南昌大学第一附属医院泌外研究所完成。材料:健康雄性SD大鼠5只,由江西中医学院实验动物中心提供。生物可降解聚酯材料为本项目小组正在研制的一种新的脂肪族/芳香族共聚脂。方法:通过溶液浇铸法将可降解聚酯(聚对苯二甲酸双酚A酯-co-对苯二甲酸己二酯-co-乳酸)材料制备成共聚酯生物膜,同法制备聚氯乙烯生物膜。将共聚酯生物膜消毒后浸于细胞培养基中,收集材料浸提液,过滤灭菌保存,同法制备聚氯乙烯浸提液。取第三四代大鼠骨髓间充质干细胞,以2×107L-1密度接种于96孔板中,以1.3×105个/孔接种于含上述制备的无菌共聚脂生物膜的24孔板内。分为3组:①阴性对照组,单纯加入DMEM培养基。②实验组,在加入DMEM培养基的基础上,分别加入12.5%,25%,50%,100%的材料浸提液。③阳性对照组,在加入DMEM培养基的基础上,加入聚氯乙烯浸提液。主要观察指标:通过中性红摄取法、碱性品红染色法、MTT还原检测法,对材料毒性进行评价,电镜观察细胞在生物膜上的生长情况。结果:培养1,3,5,7d后,阳性对照组细胞活力及代谢能力有不同程度的下降(11%～16%);实验组与阳性对照组吸光度值比较差异有显著性意义(P<0.001),随培养时间延长,细胞活力、数量及代谢能力均稍有增强(1%～4%);实验组与阴性对照组吸光度值比较无明显差异(P>0.05)。电镜扫描及吖啶橙/溴化乙啶染色结果显示,接种第1天骨髓间充质干细胞即�BACKGROUND： Aromatic polyester can not be used alone as degradable material due to its poor biodegradation. In the School of Materials Science and Engineering, Nanchang University, aromatic/aliphatic copolyesters, a new kind of degradable biomaterial is synthesized by melt co-polycondensation and transesterification of terephthalyl chloride, bisphenol A, hexanediol as well as low lacticacid polymer, which has been patented. OBJECTIVE： To study the biocompatibility between aromatidaliphatic copolyesters and bone marrow mesenchymal stem cells （BM-MSCs） in rats. DESIGN,TIME AND SETTING： An in vitro cellular-materials experiment. The experiment was performed at the Institute of Urology, the First Affiliated Hospital of Nanchang University between April 2006 and April 2007. MATERIALS： Five healthy, adult, male, Sprague Dawley rats were provided by the experimental animal center of Jiangxi University of Traditional Chinese Medicine. The bio-degradable polyester was aromatic/alfphatic copolyesters prepared by our group. METHODS： A new degradable biomaterial poly （4,4＇-isopropylidenediphenyl terephthalate）-co-poly（hexylene terephthalate）-co-polylactide （PBHTL） was prepared into biomembrane by casting method. Simultaneously, polyvinyl chloride biomembrane was prepared with the same method. The leaching liquor of biomembrane was collected when the biomembranes were sterilized and immersed in culture medium. The BM-MSCs of rats with the 3^rd or 4^th passage were incubated at the 96-well plates with density of 2×10^7/L and at 24-well plate with 1.3 × 10^5 per well. The experiment divided into 3 groups. In the negative control group, cells were cultured with DMEM. In the experimental group, 12.5%, 25%, 50%, 100% material leaching liquor were additional added based on DMEM. In the positive group, polyvinyl chloride leaching liquor was added except DMEM. MAIN OUTCOME MEASURES： The cytotoxicity of material was evaluated by neutral red uptake assay, basic fuchsin staining and MIT method. Growth of

关 键 词：芳香/脂肪共聚酯 生物材料 组织工程 细胞相容性 

分 类 号：R318[医药卫生—生物医学工程]