实验性自身免疫性脑脊髓炎小鼠脾脏和肝脏中NKT细胞调变的研究  

The study of changes on NKT cells of experimental autoimmune encephalomyelitis (EAE) mice

作　　者：欧阳清[1] 陈琨[2] 王曦[2] 张春梅[3] 郭俊[4] 魏玉英[3] 孙元杰[3] 徐竹蔚[3] 杨琨[3] 

机构地区：[1]第四军医大学口腔医学系6队 [2]第四军医大学口腔医学系9队 [3]第四军医大学基础部免疫学教研室 [4]第四军医大学唐都医院神经内科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2009年第10期894-896,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家高技术研究发展计划(863)资助项目(2006AA02A237);国家自然科学基金资助项目(30872371);第四军医大学学员课外科研课题(2008年)

摘　　要：目的:观察NKT细胞在实验性自身免疫性脑脊髓炎(EAE)小鼠脾脏和肝脏中所占百分比的变化特点,探讨NKT细胞在EAE模型中的免疫调节作用。方法:以MOG35-5521肽诱导C57BL/6小鼠建立EAE模型并进行临床评分。于发病高峰期处死小鼠,分离脾脏和肝脏淋巴细胞,采用免疫荧光染色和流式细胞术(FCM)分析,观察EAE小鼠与正常小鼠脾脏和肝脏中NKT细胞在全部淋巴细胞中所占百分率的变化。结果:在EAE小鼠不同器官中,NKT细胞占淋巴细胞的百分率均较正常小鼠减少。脾脏NKT细胞百分率(%)从正常组2.22±0.14下降到EAE模型组1.94±0.07(P<0.05),肝脏NKT细胞百分率(%)从正常组5.52±2.17下降到2.67±1.41(P<0.05)。结论:NKT细胞在EAE模型C57BL/6小鼠脾脏和肝脏中增殖受抑,提示EAE发病可能通过对NKT细胞数量的调节进而影响其对免疫应答的调节。AIM： To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis （EAE）, and to study the role NKT cells play in the immunoregulation of EAE. METHODS： C57BL/6 mice were immunized with MOG35-55 peptide and received clinical evaluation daily. The mice were sacrificed at the fastigium and the splenic and hepatic lymphocytes were isolated. The changes of NKT cells in normal and EAE C57BL/6 mice were detected by flow cytometry. RESULTS： The percent of NKT cells in lymphocytes of different organs of EAE model were greater decreased than in that of normal mice. The percent of NKT cells in splenic lymphocytes of normal mice was 2. 22 ± 0.14, while that in EAE mice was 1. 94 ± 0.07 （ P 〈 0.05 ）. The percent of NKT cells in hepatic lymphocytes of normal mice was 5.52 ± 2.17, while that in EAE mice was 2, 67 ± 1.41 （ P 〈 0. 05）. CONCLUSION： The proliferation of splenic and hepatic NKT cells in C57BL/6 mice are inhibited in EAE model, which may indicate that the immune function conducted by NKT cell is down regulated in EAE mice.

关 键 词：NKT细胞 EAE 免疫调节 

分 类 号：R392.12[医药卫生—免疫学]