测定超氧化物歧化酶活性的一种新的极谱分析方法  

A Polarography for the Determination of Superoxide Dismutase Activity

作　　者：杨天鸣[1] 黎瑞珍[1] 陆俭洁[1] 

机构地区：[1]中南民族学院化学系,武汉430074

出　　处：《分析化学》1998年第11期1342-1345,共4页Chinese Journal of Analytical Chemistry

摘　　要：报道一种测定超氧化物歧化酶（ＳＯＤ）活性的新的极谱分析方法。在０．１２５ ｍｏｌ／ＬＮＨ３·Ｈ２Ｏ－０．７５ ｍｏｌ／Ｌ　ＮＨ４　Ｃｌ－２．５％ ＮａＳＯ３－０．５％吐温－８０（ｐＨ＝ ８．８± ０．３）的介质中，ＳＯＤ在－０．５２Ｖ（νｓＳＣＥ）处产生一氧极谱催化波。ＳＯＤ在１．０ × １０３ Ｕ／Ｌ～４．０ ×１０３ Ｕ／Ｌ的活性含量范围内与催化电流呈线性关系，检出限为８．０×１０２ Ｕ／Ｌ。用ＳＯＤ标准溶液进行标准加入回收测定，平均回收率为９９．６％，ＲＳＤ为０．３％。本方法具有灵敏度高、重现性好、试剂易得、使用安全等特点。A new polyrography for the determination of superoxide dismutase activity was developed. In the medium of 0. 125 mol/L NH3. H2O, 0. 75 mol/L NH4 Cl, 2. 5% Na2SO3 and 0. 5%Tween-80(pH = 8. 8 ± 0. 3), a polarographic catalytic wave of oxygen proceed from superoxide dismutase was observed. The peak potential is - 0. 52 V(vs. SCE). The linear relationship between catalytic current and the activity of superoxide dismutase is from 1 .0 × 103 U/L to 4 .0× 103 U/L. The detection limit is 8 .0× 102 U/L. The recovery is 99.6%. The relative standard deviation (n = 6) is 0. 3%. This method has the advantages of high sensitivity, good reproducibility, and the reagent is accessible.

关 键 词：超氧化物歧化酶 活性测定 极谱 分析 

分 类 号：Q554.903[生物学—生物化学]