可控轴向压力致兔腰椎间盘退变模型的建立及评价(英文)  

Development and evaluation of controllable axial loading-induced rabbit lumbar disc degeneration model

作　　者：熊蠡茗[1] 邵增务[1] 郭兵[1] 杨述华[1] 裴洪[1] 詹子睿[1] 俞旭东[1] 王河忠[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,中国武汉430022

出　　处：《中国矫形外科杂志》2009年第19期1492-1496,共5页Orthopedic Journal of China

基　　金：Supported by:a grant from the National Natural Sciences Foundation of China(No.30700841)

摘　　要：[目的]建立可控轴向压力诱发的椎间盘退变动物模型并考察其特性。[方法]取4个月龄日本大白兔40只,将其随机分为5组(每组8只)。采用基于横穿椎体的克氏针的自制加压装置,分别对2～4组动物腰椎间盘施加10 kg压力1、2、4周及8周,第1组作为假手术对照组。Thompson分级法及兔腰椎间盘磁共振(MRI)评价退变程度,HE染色及Ⅰ、Ⅱ型胶原免疫组化染色考察其组织学改变。另外构建纤维环损伤致兔腰椎间盘退变模型,并对标本行HE染色以供比较。[结果]①Thompson分级:第2组7只均为Ⅱ级,第3组6只Ⅲ级,第4组5只为Ⅳ级,第5组4只动物退变程度达到V级。②MRI评分显示,第2组椎间盘以轻度退变为主;第3组椎间盘主要为中度退变。第4组、第5组则以重度退变为主。③HE染色显示,该模型椎间盘各区域的退变情况较纤维环损伤模型更为均匀。④免疫组化染色显示,退变过程中椎间盘基质成分退化的趋势与人体相符。[结论]可控轴向压力致兔腰椎间盘退变模型可以高效诱发椎间盘退变,尤其在轻、中度椎间盘退变方面具有优势。该模型可供深入研究,并用于椎间盘退变的治疗研究。[ Objective] To develop a new rabbit lumber disc degeneration model by imposing controllable axial olading upon corresponding segment, and to evaluate the features of the new model. [ Method ] Forty Japanese white rabbits （4 months old） were randomly divided into groups 1, 2, 3, 4 and 5 （8 rabbits in each group） . A self - made loading device based on 2 K - wires that punctured through the adjunctive vertebras of corresponding intervertebral discs, was utilized to impose 98 N pressure on the discs for 1,2, 4 and 8 weeks in groups 2, 3, 4 and 5 respectively. And no pressure was imposed on the group 1 as sham - operated group （ control group） . Degeneration of the discs was evaluated by Thompson＇ s grading system, magnetic resonance imaging （MRI） scanning, haematoxylin and eosin （HE） staining, and immunohistochemical staining for type I and type II collagen. [ Result] （ 1 ） Aceording to the Thompson＇ s system, 7 discs reached grade I in group 2, 6 discs reached grade III in group 3, 5 discs reached grade IV in group 4, and 4 discs reached grade V in group 5, respectively. （2） MRI scan displayed that the discs in group 2 demonstrated mile degeneration, and those in group 3 moderate degeneration, while the discs in groups 4 and 5 revealed severe degeneration. （3） HE staining indicated that the changes of disc degeneration exhibi- ted more homogeneous than those of annulus fibrosus injury model. （4） Immunohistochemical staining showed that the trend of matrix degeneration was similar to that of human disc degeneration. [ Conclusion] The new model is capable of simulating lum- bar disc degeneration effectively, especially for establishment of mild or moderate degeneration model. This model can be used in studies on the mechanism and therapy of intervertebral disc degeneration.

关 键 词：椎间盘退变 兔 模型 动物 压力 

分 类 号：R681.53[医药卫生—骨科学] R-332[医药卫生—外科学]