乙基紫标记分光光度法测定脱氧核糖核酸  

Spectrophotometric Determination of Deoxyribonucleic Acid Labeling With Ethyl Violet

作　　者：李天剑[1] 沈含熙[1] 罗云敬[1] 

机构地区：[1]南开大学化学系,天津300071

出　　处：《分析化学》1998年第11期1372-1375,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!(29675009)

摘　　要：研究了阳离子染料乙基紫与脱氧核糖核酸（ＤＮＡ）的结合反应。在ｐＨ＝６．４～７．４的条件下，乙基紫在５９５ｎｍ处有最大吸收峰，当加入ＤＮＡ后，乙基紫的吸收峰显著下降，而在５０７ｎｍ处出现新的吸收峰。以乙基紫为标记物，根据其５９５ ｎｍ吸收峰下降的程度，可用于ＤＮＡ的定量测定。ＤＮＡ的线性响应范围为０～１．０８ × １０－５ ｍｏｌ／Ｌ；表观摩尔吸光系数ε５９５＝７．２４ ×１０４ Ｌ·ｍｏｌ－１·ｃｍ－１。方法具有较高的灵敏度和选择性。用于合成试样分析，结果满意。The binding reaction of DNA (calf thymus deoxyribonucleic acid) with dye ethyl violet has been studied. In the medium of pH range 6 .4 - 7. 4, ethyl violet showed a decrease in absorption at the wavelength of its maximal absorption peak (595 um) on binding to DNA, and at the same time a new peak of the binding product appeared at 507 um. A novel method for the determination of DNA at 595 um using ethyl violet as a labeling agent was developed. The method is of high sensitivity as well as selectivity, and has been used to determine DNA in synthetic Samples with satisfactory results.

关 键 词：乙基紫 分光光度法 DNA 结合反应 分析 

分 类 号：Q523.03[生物学—生物化学]