苦楝和杉木的种间化感作用研究  

Study on Allelopathy of Melia azedrach and Chinese-fir

作　　者：杜玲[1] 曹光球[2] 林思祖[2] 

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [2]福建农林大学林学院,福州350002

出　　处：《科学技术与工程》2009年第20期5982-5986,共5页Science Technology and Engineering

基　　金：福建省自然科学基金项目(B0010020)资助

摘　　要：采用正交试验方法和超临界流体萃取法,研究了苦楝叶提取物对杉木的化感作用。生物检测结果显示,与酒精对照相比:萃取参数1、4和7等条件苦楝叶提取物对杉木种子绝对发芽率、绝对发芽势、胚根长、胚轴长、鲜重和干重等发芽指标均表现出不同程度的促进作用,萃取参数2和3等条件苦楝叶提取对除胚根和胚轴外的各发芽指标起促进作用,萃取参数5条件苦楝叶提取物对除鲜重和干重外的各发芽指标起促进作用,萃取参数6条件苦楝叶提取物对除胚根和鲜重外的各发芽指标起促进作用,而萃取参数8条件苦楝叶提取物仅对绝对发芽势起促进作用。同一参数条件三种浓度相比:萃取参数1、4、5和7条件下以100 mg/kg浓度苦楝叶提取物处理的对杉木种子各发芽指标促进作用最明显,接着为50 mg/kg或200 mg/kg浓度处理的;萃取参数2和3条件下以200 mg/kg浓度提取物处理的促进作用最强,接着为50 mg/kg或100 mg/kg浓度处理的。Melia azedrach leaves extracts through supercritical fluid extract under orthogonal experiment affected the Chinese fir seeds germination differently. Bioassay results revealed： compared with alcohol control, extracts at parameter 1,4, 7 stimulated the absolute germination rate and potential, radicle growth, plumular axis growth, fresh weight, dry weight at different levels; extracts at parameter 2, 3 stimulated the all germination index except radicle growth and axis growth; extracts at parameter 6 stimulated all but radicle growth and fresh weight; extract at parameter 8 stimulated only the germination potential. Among the three concentrations at the same parameter, extract of 100 mg/kg at parameter 1,4, 5, 7 stimulated the germination most, the following were 50 mg/kg and 200 mg/kg; extract of 200 mg/kg stimulated most , the following were 50 mg/kg and 200 mg/kg.

关 键 词：苦楝 杉木 超临界流体萃取 提取物 化感作用 

分 类 号：S792.5[农业科学—林木遗传育种]