核酸对邻菲啰啉的荧光猝灭及其分析应用  

The Quenching of o-Phenanthroline Fluorescence by Nucleic Acids and Its Analytical Applications

作　　者：魏亦男[1] 李元宗[1] 常文保[1] 慈云祥[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《分析化学》1998年第10期1178-1182,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!(29575191);国家教委<跨世纪优秀人才计划>基金

摘　　要：邻菲啰啉受到 ２３０ｎｍ及２６７ ｎｍ紫外光激发，在３６７ ｎｍ处产生一荧光峰。而天然和热变性鱼精子脱氧核糖核酸以及酵母核糖核酸的加入会猝灭邻菲啰琳的这一荧光发射。实验表明，该体系可在较宽的范围内灵敏地测定核酸。When excited with ultraviolet light of 230 nm or 267 nm, o-phenanthroline can emit fluorescence at 367 urn. In the pH around 6. 2, nucleic acids, including fish sperm deoxyribonucleic acid (DNA), thermally denatured fish sperm DNA and yeast ribonucleic acid (RNA), can quench this fluorescence emission without causing deformation or shift of the excitation and emission peaks. This reaction can be used to determine the nucleic acids in solution sensitively and in a comparably wide range of concentration.

关 键 词：核酸 邻菲LUO啉 荧光分析 分析 

分 类 号：Q520.3[生物学—生物化学] O626.321[理学—有机化学]