玻璃载体表面脱氧核糖核酸的固定及其化学发光检测  

Attachment of Deoxyribonucleic Acid to Glass Supports and Its Detection by Chemiluminescence

作　　者：张国军[1] 周宜开[1] 袁津玮[1] 柏正武[1] 王洪君[1] 郝巧玲[1] 

机构地区：[1]同济医科大学环境医学研究所,武汉430030

出　　处：《分析化学》1998年第10期1205-1208,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：用硅烷化偶联剂把ＤＮＡ直接共价固定在载玻片表面，将辣根过氧化物酶标记的探针与之进行核酸杂交，杂交后用增强的化学发光检测。方法的检出限为７５ｐｇ。研究了ＤＮＡ分子固定在玻璃载体表面的各种条件，并建立了在玻璃载体表面进行核酸杂交的体系，为研制光纤ＤＮＡ生物传感器打下了基础。Deoxyribonucleic acid (DNA) was immobilized directly on the surfaces of glass supperts using silane coupling agent, then hybridized it with horseradish peroxidase-labeled probe and detected by enhanced chemiluminescence. The detection limit of the projposed method is 75 pg. Several experimental conditions of DNA attached to glass supports and the hybridization procedure of nucleic acid were investigated.These will offer the basis for the research of fiber-optic DNA biosensor.

关 键 词：DNA 固定 玻璃载体 化学发光 分析 生物传感器 

分 类 号：Q523.03[生物学—生物化学]