二乙烯苯与乙烯基吡咯烷酮共聚硅质填料的制备及对蛋白质的分离  

Divinylbenzene-Vinylpyrrolidone Copolymer Silica Packing for Chromatographic Separation of Proteins

作　　者：杨炳华[1] 樊立民[1] 魏芸[1] 沈润南[1] 蒋生祥[1] 陈立仁[1] 

机构地区：[1]中国科学院兰州化学物理研究所,兰州730000

出　　处：《分析化学》1998年第10期1267-1270,共4页Chinese Journal of Analytical Chemistry

摘　　要：大孔硅胶基质与二乙氧基甲基乙烯基硅烷反应后，再与二乙烯苯、乙烯基吡咯烷酮共聚，制得新型反相色谱填料。实验结果表明，该填料对蛋白质的分离性能好、柱效高、速度快、惰性好。为生物大分子的高效液相色谱分离提供了一种新型填料。A novel reversed-phase chromatographic packing for protein separation is described. First diethoxymethylvinylsilane was bonded to silica gel, then the divinylbenzene (DVB) and vinylpyrrolidone (VPD) were copolymerized on the silica gel to produce a packing with network - structured surface and retains the wide-pore structure of the matrix.Good selectivity and separation ability of the synthesized packing toward proteins were demonstated in the reversed-phase chromatographic mode,Seven proteins attained baseline separation within 18 min. It is able to separate biopolymer quickly.

关 键 词：反相色谱 填料 大孔硅胶 蛋白质 分离 固定相 

分 类 号：O657.703[理学—分析化学] Q510.3[理学—化学]