珍珠黄杨无性系过氧化物酶同工酶分析研究  

Peroxidase Isozyme Study of Buxus sinica var. parvifolia Clones

作　　者：吕乐燕[1] 季孔庶[2] 

机构地区：[1]江苏畜牧兽医职业技术学院园林科技系,江苏泰州225300 [2]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《安徽农业科学》2009年第30期14600-14601,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]利用过氧化物酶(POD)同工酶标记技术对珍珠黄杨无性系进行研究。[方法]以珍珠黄杨普通无性系和新整理出的5个无性系为材料,对其叶片提取液进行POD同工酶电泳。[结果]结果表明:珍珠黄杨各无性系经POD同工酶电泳后条带数较少,只有4条,多态性不强,但根据酶谱染色深浅仍能区别各个无性系。[结论]研究认为该技术在珍珠黄杨无性系鉴定中有一定的应用潜力。[ Objective ] The research aimed to study the peroxidase isozyme patterns of Buxus sinica var. parvifolia by polyacrylamide electrophoresis. [ Method] Taken common clones and five new clones of B. sinica var. parvifolia for materials,and its leaves extract was used to carry out peroxidase isozyme electrophoresis. [ Result ] The results showed that only 4 isozyme bands were found in B. sinica var. parvifolia, and the polymorphism of them was not significant. But each clone could be distinguished from the degree of dyeing. [ Conclusion] It was considered in this study that peroxidase isozyme analysis was an efficient method for cuhivar identification of B. sinica var. parvifolia clones.

关 键 词：珍珠黄杨 过氧化物酶同工酶 无性系鉴定 

分 类 号：S718.43[农业科学—林学]