印楝(Azadirachta indica A.)高频植株再生系统的建立  

Establishment of Regeneration System for High Frequency Plantlet of Azadirachta indica A. Juss

作　　者：王颖[1] 范春丽[2] 杨光伟[3] 

机构地区：[1]茂名学院化学与生命科学学院,广东茂名525000 [2]郑州师范高等专科学校生命科学系,河南郑州450044 [3]西南大学生物技术中心,重庆400716

出　　处：《安徽农业科学》2009年第30期14616-14617,共2页Journal of Anhui Agricultural Sciences

基　　金：科技部重点科技项目(K99-09-11)

摘　　要：[目的]建立印楝高频植株再生系统。[方法]以印楝带芽茎段为外植体,以MS为基本培养基,分别附加6-BA0.1、0.3、0.5、0.8、1.0mg/L和NAA0、0.1、0.3、0.5mg/L组配14个培养基配方处理进行芽分化的诱导培养试验,生根培养采用MS+IBA0.2mg/L、1/2MS和MS的3种培养基,研究外植体最适宜的消毒时间和取材部位,筛选诱导印楝芽分化和生根的最佳培养基配方。[结果]初代培养中,印楝外植体最适宜的消毒时间为10～12min,最适宜的取材部位为茎尖及植株的上部。14个培养基处理中,诱导芽分化的最佳培养基为:MS+6-BA0.5mg/L+NAA0.1mg/L+0.65%琼脂+3%蔗糖(pH5.8)。诱导生根的最佳培养基为:1/2MS+0.65%琼脂+3%蔗糖(pH5.8),生根率85.13%,小苗移栽成活率80%。[结论]该研究为印楝规模化快繁和遗传转化体系的建立奠定了基础。[ Objective ] The study aimed to establish the regeneration system for high frequency plantlets of Azadirachta indica A, Juss. [ Method] Taking A. indica stem segments with buds as the tested explants, MS as the basic medium, 14 treatments of medium formulas were matched by adding 6-BA 0.1,0.3, 0.5, 0.8, 1.0 mg/L and NAA 0, 0.1,0.3, 0.5mg/L to make the culture test of inducing the bud differentiation. 3 kinds of media of MS ＋ IBA 0.2 mg/L, 1/2MS and MS were used to make the rooting culture. The optimum sanitizing time and the material parts for the explants were studied and the optimum medium formulas for inducing the bud differentiation and rooting of A. indica were screened. [ Result] In initial culture, the optimum sanitizing time for the explants of A. indica was l0 - 12 min and the optimum material parts were shoot tip and the top of the plant. Among 14 medium treatments, the optimum medium for bud differentiation was MS ＋ 0.5 mg/L 6-BA ＋0.1 mg/L NAA ＋ 3% sucrose （pH 5.8） ＋ 0.65% agar and the optimum rooting medium was 1/2 MS medium ＋ 3% sucrose ＋ 0. 65% agar （ pH 5.8 ）, with rooting rate of 85.13% and the survival rate of 80% for the transplanted plantlets. [ Conclusion] This study laid the foundation for the massive micro-propagation and genetic transformation of A. indica.

关 键 词：印楝 组织培养 快繁 

分 类 号：S722.37[农业科学—林木遗传育种]