中草药与卵黄抗体复合气雾剂体外抑制病毒试验  

Restraining Virus Experiment of Complex Aerosol of Chinese Herbal Medicine and Yolk Antibody in Vitro

作　　者：朱达文[1] 王冬梅[1] 王寿轩[2] 朱本康[2] 刘俊栋[3] 方希修[1] 朱涛[2] 

机构地区：[1]江苏畜牧兽医职业技术学院饲料与营养研究所,江苏泰州225300 [2]江苏省徐州金牌药业有限公司,江苏徐州221600 [3]江苏畜牧兽医职业技术学院兽医系,江苏泰州225300

出　　处：《安徽农业科学》2009年第30期14715-14716,共2页Journal of Anhui Agricultural Sciences

基　　金：江苏省科技攻关项目(BE2003618)

摘　　要：[目的]将不同配比的中草药与卵黄抗体复合气雾剂体外抑制病毒的能力进行比较,以确定中草药与卵黄抗体的最佳配比。[方法]试验样品为不同配比的中草药"禽喘康复散"与卵黄抗体复合气雾剂,配比分别为1:1.0、1:0.8、1:0.5、1:0.2,分别记为样品Ⅰ、Ⅱ、Ⅲ、Ⅳ。采用病毒致细胞病变抑制试验和血凝抑制试验,测定样品抑制病毒的能力。[结果]结果表明,样品I对腺病毒7型(AdV7)的最小抑制浓度为1/640g/ml,作用弱于样品II,而对其他病毒的作用都最强。样品IV除对腺病毒3型(AdV3)的最小抑制浓度为1/640g/ml,对其余病毒的抑制作用在4种样品中最弱。体外抑制病毒试验表明中草药与卵黄抗体配比为1:1.0对各病毒的抑制效果最好。[结论]选用1:1.0配比的复合气雾剂最能有效防治呼吸道疾病。[ Objective ] The research aimed to compare restraining abilities on virus in vitro of different complex aerosol of Chinese herbal medicine and yolk antibody, and to establish the best ratios of Chinese herbal medicine and yolk antibody. [ Method ] Taken different mixture ratios of Chinese herbal medicine avian pant recovery powder and yolk antibody complex aerosol as materials, and the ratios of 1 ： 1.0,1 ： 0.8,1 ： 0. 5,1 ： 0.2 were recorded as sample Ⅰ , Ⅱ, Ⅲ and Ⅳ. Cytopathic inhibition test of different virus and the inhibition test of TPHA were carried out to determine the abilities of virus inhibition. [ Result ] The results showed that the minimum virustatic concentration of sample Ⅰ for AdV7 is 1/640 g/m;, which is less than sample Ⅱ, but for other virus is strongest. Other than the minimum virustatic concentration for AdV3 is 1/640 g/ml, the inhibition to other virus of sample Ⅳ is weakest among four samples. The experiments of virustatic in vitro suggest that compound aerosol with ratio of Chinese herbal medicine and yolk antibody 1：1.0 has best inhibitory activity on different virus. [ Conclusion] Ratio of 1： 1.0 compound aerosol had the best effects on the control of breath illness.

关 键 词：中草药 卵黄抗体 气雾剂 抑病毒 

分 类 号：S853.7[农业科学—临床兽医学]