HPLC-MS法测定人血浆中依普利酮浓度  

Determination of eplerenone in human plasma by HPLC-MS

作　　者：赵越平[1] 陈苏宁[1] 丁莉坤[1] 杨志福[1] 王志睿[1] 金鑫[1] 文爱东[1] 

机构地区：[1]第四军医大学西京医院药剂科,陕西西安710033

出　　处：《第四军医大学学报》2009年第18期1828-1830,共3页Journal of the Fourth Military Medical University

摘　　要：目的:建立人血浆中依普利酮浓度的HPLC-MS测定法.方法:血浆样品中加入内标溶液后,以液-液萃取方法进行样品处理,采用HPLC-MS方法测定其血药浓度.结果:标准曲线线性范围为2.080～4160μg/L,标准曲线线性关系良好,回归方程为:f=0.0005520+0.001333×C(r=0.9984).高、中、低3浓度的批内和批间变异均小于15%,提取回收率在73.6%～75.5%.结论:建立人血浆中依普利酮浓度的HPLC-MS测定法操作简便,灵敏度高,适用于依普利酮的血药浓度检测及药动学研究.AIM：To develop an HPLC-MS method for the determination of Eplerenone concentration in human plasma. METHODS： The samples were extracted with ethyl acetate after adding the internal standard solution, and analytes were detected by mass spectrometer in positive models. RESULTS： The standard curve was linear in the range of 2. 080 - 4160 μg/L and the mean calibration curve： f= 0. 0005520 ＋ 0. 001333 × C （r = 0.9984）. The relative standard derivation of intra- and inter-run was all less than 15%. The extraction recoveries of eplerenone were between 73. 6% - 75. 5%. CONCLUSION： A specific, sensitive and simple method has been established, which is good for determination of eplerenone concentration in human plasma and for pharmacokinetic studies.

关 键 词：依普利酮 光谱分析 质量 色谱法 高压液相 血药浓度 

分 类 号：R917[医药卫生—药物分析学]