2型糖尿病患者红细胞醛糖还原酶水平与血清氧化应激状态的关系  

The relationship between AR level of erythrocyte and serum oxidative stress in patients with type 2 diabetes

作　　者：葛震坤[1] 

机构地区：[1]安徽省含山县人民医院内科,安徽含山238100

出　　处：《中国医药导报》2009年第29期20-22,共3页China Medical Herald

摘　　要：目的:探讨2型糖尿病患者红细胞中醛糖还原酶(aldose reductase,AR)的含量与氧化应激指标超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、丙二醛(malondialdehyde,MDA)水平改变之间的联系。方法:采集25例2型糖尿病患者及25例健康人血样作正常对照,以荧光法检测红细胞中的AR水平,以ELISA法检测血清SOD、GSH-PX活性和MDA含量。结果:2型糖尿病组血清SOD、GSH-PX活性分别为(0.85±0.06)μg/ml和(126.98±1.11)μg/ml,较正常对照组[分别为(12.94±0.11)μg/ml和(298.29±2.56)μg/ml]显著下降(P<0.05),而血清MDA含量则从(1.68±0.05)μg/ml上升至(24.98±2.13)μg/ml(P<0.05),提示2型糖尿病患者的外周血存在氧化应激状态。与此相对应,2型糖尿病患者红细胞中的AR含量为(739.8±80.2)U/gHb,较正常对照组水平[(140.5±26.7)U/gHb]明显升高(P<0.05),其上调表达与氧化应激状态呈正相关。结论:红细胞中AR表达的上调与糖尿病患者体内的氧化应激关系密切,可能通过该环节参与2型糖尿病病变的发生和发展。Objective： To observe the relationship between the content of aldose reductase （AR） in erythrocyte of patients with type 2 diabetes and serum levels of superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） as well as malondialdehyde （MDA）, which are biomarkers for oxidative stress response. Methods： The whole bloods were collected from 25 cases of patients with type 2 diabetes. Fluorescence method was applied to detect the AR level in erythrocytes, and ELISA method was performed to analyze the serum levels of SOD, GSH-PX and MDA. 25 blood samples from healthy volunteers were treated in the same way and selected as control. Results： The serum activities of SOD and GSH-PX in diabetes group were （0.85±0.06）μg/ml and （126.98±1.11）μg/ml respectively, showed a remarkable decrease （P〈0.05） compared with those of the healthy control group of （12.94±0.11）μg/ml and （298.29±2.56）μg/ml respectively. While the serum level of MDA increased significantly from （1.68±0.05）μg/ml to （24.98±2.13）μg/ml （P〈0.05）, indicating an increasing state of oxidative stress in peripheral blood of patients with type 2 diabetes. At the same time, a remarkable increase of AR level in erythrocytes of （739.8±80.2）U/g Hb of type 2 diabetes was observed, which was significantly higher than that of control group [（140.5±26.7） U/g Hb] （P〈0.05）, and the up-regulation of AR expression in erythrocytes had a positive relation with oxidative stress state in peripheral blood. Conclusion： The up-regulated expression of AR in patients with type 2 diabetes is closely associated with oxidative stress state, and may be involved in the occurrence and development of type 2 diabetes through oxidative pathway.

关 键 词：糖尿病 氧化应激 醛糖还原酶 

分 类 号：R587.1[医药卫生—内分泌]