hTERC基因的FISH检测对良恶性腹水鉴别诊断的价值  

The differential diagnostic value of hTERC oncogene in benign and malignant ascites by fluorescence in situ hybridization

作　　者：齐丽敏[1] 凌斌[1] 李俊[2] 刘巧[1] 冯定庆[1] 周颖[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科,安徽省立医院妇产科分子实验室,合肥230001 [2]安徽医科大学第一附属医院肿瘤内科细胞室,合肥230022

出　　处：《安徽医科大学学报》2009年第5期554-557,共4页Acta Universitatis Medicinalis Anhui

基　　金：卫生部医药卫生科技发展中心项目(编号:WKJ2007-3-001)

摘　　要：目的检测腹水脱落细胞端粒酶(hTERC)基因的状态,以探讨其在良恶性腹水鉴别诊断中的价值。方法采用荧光原位杂交(FISH)方法,检测26例恶性腹水和15例良性腹水(对照组)脱落细胞hTERC基因的状态。结果①hTERC基因在良性腹水和恶性腹水中的扩增阳性率分别为6.7%和88.5%,恶性组显著高于良性组(P<0.05)。②恶性腹水中,FISH检测阳性率88.5%明显高于细胞学检测阳性率46.2%(P<0.05);良性腹水中,FISH诊断特异性略低于细胞学检查结果(93.3%和100%),但无统计学意义(P>0.05)。③不同肿瘤引起的腹水hTERC基因扩增阳性率比较差异无统计学意义(P>0.05)。结论恶性腹水hTERC基因高度扩增,检测该基因对良恶性腹水有重要鉴别诊断价值,尤其对细胞学检查阴性的患者。Objective To investigate the state of hTERC oncogene in ascitic exfoliative cells, and to evaluate the effect and clinical significance of hTERC in benign and malignant ascitcs. Methods The fluorescent in situ hybridization （FISH） was used to detect the state of hTERC oncogene in 26 patients with various malignant ascites and 15 with benign ascites. Results （1） Positive amplification rates of hTERC in benign and malignant ascites were 6.7% and 88.5 % , respectively. The amplification in benign ascites were lower than that in malignant ascites （P 〈 0.05 ）. （2） In malignant ascites ,the positive rates by cytology （46.2%） were lower than those by FISH（88.5% ） （P 〈 0. 05 ）;In benign ascites, the specificity of diagnosis by FISH was slightly lower than that by cytology result （93.3% and 100% ）, but there was no significant difference （P 〉 0. 05 ）. In addition, the positive amplification rate of the hTERC gene were no significant difference between malignant ascites （P 〉 0. 05 ）. Conclusions hTERC gene is high amplification in malignant ascites, and it may be useful in differential diagnosis of benign and malignant ascites, particularly in cases of negative cytology.

关 键 词：原位杂交 荧光 端粒末端转移酶 腹水/诊断 

分 类 号：R345.9[医药卫生—基础医学] R446.142