蜂毒素的体内外抗肿瘤研究  

Experimental study of antitumor effects of melittin

作　　者：靳弟[1] 李俊[1] 吴宝明[1] 王怡苹[1] 赵斌[1] 

机构地区：[1]安徽医科大学药学院,合肥230032

出　　处：《安徽医科大学学报》2009年第5期590-593,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的研究蜂毒素(melittin)在体内、外对H22细胞的增殖抑制作用,初步探讨其机制。方法通过四甲基偶氮唑盐比色法(MTT法)检测melittin对H22细胞的增殖抑制情况;腹腔注射浓度为1、2、4 mg/(kg.d)的melittin,给药10d,观察melittin对荷瘤小鼠的肿瘤抑制以及机体免疫变化情况。结果melittin在体外对H22细胞有明显的抑制作用;体内实验表明浓度为2、4 mg/(kg.d)的melittin能够增强机体免疫力,对荷瘤小鼠肿瘤有着较强的抑制作用,且效果随剂量的增大而增强。结论melittin在体外、体内均对H22细胞有着较强的抑制作用,可能通过增强机体免疫而产生作用。Objective in vivo and in vitro an tected by MTr assay; was administered at d To study the effects of melittin on the growth and d investigate the anti the changes of tumo oses 1.0,2. 0 and 4. -tumor mechanisms. Methods proliferation of H 22 hepatocarcinoma cells The growth and proliferation rate was der and immunity of H22 tumor-burdened 0 mg/（kg · d） for 10 days. Results mice were observed after melittin There was apparent inhibition of melittin on H22 cells ; The results of experiment in vivo showed that melittin at doses 2. 0 and 4. 0 mg/（ kg · d） could inhibit the tumor growth,reinforced the immunity of mice. Conclusion Melittin can inhibit the proliferation of H22 cells both in vitro and in vivo. Maybe melittin inhibits the tumor growth through reinforcing the immuntity of mice.

关 键 词：蜂毒肽/药理学 抗肿瘤药 

分 类 号：R996.3[医药卫生—毒理学] R735.7[医药卫生—药学]