系统性红斑狼疮患者白介素-10mRNA、白介素-18mRNA、FasmRNA表达水平的研究  

Study on Expression of Interleukin-10 mRNA,Interleukin-18m RNA and Fas mRNA in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus

作　　者：宫泽琨[1] 张峻岭[1] 

机构地区：[1]天津市长征医院,天津300120

出　　处：《中国中西医结合皮肤性病学杂志》2009年第5期282-284,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：天津市卫生局科技基金((No200029)

摘　　要：目的探讨未经刺激外周血单一核细胞(PBMC)白介素(IL)-10 mRNA、IL-18 mRNA和Fas mRNA的表达水平在系统型红斑狼疮(SLE)发病中的作用。方法分离82例SLE患者和31例正常对照组外周血单一核细胞,提取总RNA,应用SYBR GreenⅠ实时荧光定量RT-PCR技术,检测并比较SLE患者及正常对照组PBMC中IL-10 mRNA、IL-18 mRNA、Fas mRNA表达水平的差异。结果SLE患者组PBMC IL-10 mRNA、IL-18 mRNA、Fas mRNA的表达水平明显高于正常对照组(P<0.01,P<0.05)。SLE患者组三个因子的变化趋势一致,成正相关(r=0.713,r=0.565,r=0.593,P<0.01)。结论SLE患者外周血单一核细胞存在IL-10 mRNA、IL-18 mRNA、Fas mRNA表达异常,IL-10 mRNA,IL-18 mRNA和Fas mRNA的高表达在SLE发病中起着非常重要的作用。Objective To explore the effect of reRAN expression of interleukin（IL）-10, IL-18 and Fas in unstimulated peripheral blood mononuclear ceils（ PBMCs ）in the pathogenesis of systemic lupus erythematosus （SLE）. Methods The PBMCs from 82 patients with SLE and 31 normal controls were isolated, and then the total RNA was extracted. To detect the expression of IL-10 mRNA,IL-18 mRNA and Fas mRNA in PBMCs by SYBR Green I real time polymerase chain reaction（PCR）. Results The levels of IL-10 mRNA,IL-18 mRNA and Fas mRNA in all patients were significantly higher than those in normal controls （P〈0.01 ,P〈0.05 ）. There was positive correlation between the mRNA expression of IL-10, IL-18 and Fas in patients with SLE （r=0.713,r=0.565,r=0.593,P〈0.01）. Conclusion It suggests that there are abnormalities of mRNA expression of IL-10, IL-18 and Fas in patients with SLE. The hypsi-expression of IL-10 mRNA, IL-18 mRNA and Fas mRNA has extremely important effect in the pathogenesis of SLE and relates with the activity of disease and kidney contaminate.

关 键 词：系统型红斑狼疮 IL-10 MRNA IL-18 MRNA FAS MRNA 实时荧光定量RT—PCR 

分 类 号：R593.241[医药卫生—内科学]