中国朝鲜族六个基因座遗传多态性  

Genetic polymorphisms of six X-short tandem repeats loci in Chinese Korean

作　　者：张永吉[1] 李哲 徐京男 张美花[1] 韩松瑛[1] 

机构地区：[1]延边大学基础医学院法医学教研室,吉林省延吉133000 [2]吉林天平司法鉴定中心 [3]延边州公安局刑事科学技术研究所

出　　处：《中华医学遗传学杂志》2009年第5期585-588,共4页Chinese Journal of Medical Genetics

基　　金：基金项目：国家自然科学基金（30660201）

摘　　要：目的了解中国朝鲜族群体中6个x染色体短串联重复序列（shorttandemrepeat，X—STR）基因座Dxs7132、DXS6854、DX$6769、DXS9898、DX$8378和GATA31E08在中国朝鲜族群体中的遗传多态性。方法用PCR扩增、变性聚丙烯酰胺凝胶电泳和银染方法对6个X—STR基因座进行检测。结果6个基因座中分别检出8个、6个、8个、8个、5个和10个等位基因，6个基因座基因型频率分布（女性个体）均符合Hardy-Weinberg平衡（P〉0．05）；观测杂合度均大于0．4660、多态信息含量均大于0．5293、女性个人识别力均大于0．7737、男性个人识别力均大于0．6107，男性单倍型多样性为0．9993。结论6个基因座均具有较高的遗传多态性，是较理想的遗传标记系统。为中国朝鲜族起源的研究和朝鲜族群体的亲子鉴定和个人识别提供了宝贵的基础资料。Objective To investigate the genetic polymorphisms of DXS7132, DXS6854, DXS6797, DXS9898,DXS8378 and GATA31E08 short tandem repeats （STRs） loci in Chinese Korean ethnic group of Yanbian, Jilin, and to construct a preliminary database. Methods The allele frequencies of the six STRs loci in Chinese Korean ethnic individuals were analyzed by multiplex polymerase chain reaction （PCR） and polyacrylamide gel electrophoresis （PAGE）. Results Total of 8, 6, 8, 8, 5 and 10 alleles were observed in each locus respectively. All loci （in female） met Hardy Weinberg equilibrium （P^0. 05）. The statistical analysis of the 6 STR loci showed the heterozygosities were more than 0. 4660 ,the polymorphic information contents （PIC） were more than 0. 5293, the haplotype diversity were more than 0. 9993, power of discrimination （PD） in females and males were more than 0. 7737 and 0. 6107,respectively. Conclusion The results showed that, all the 6 STR loci in this study were found to have high heterozygosity and polymorphic information content, so they could provide useful markers for genetic purposes. These results could serve as valuable data to enrich the Chinese Korean ethnic group genetic database and play an important role in genetic study of Chinese population.

关 键 词：中国朝鲜族 短串联重复序列 遗传多态性 

分 类 号：R686[医药卫生—骨科学]