RRLC-UV法同时测定丹参中酚酸类成分的含量  

Simultaneous determination of nine phenolic acids in Salvia miltiorrhiza by RRLC-UV

作　　者：王莉[1,2] 赵明波[2] 何文顺[2] 昝柯[2] 毕开顺[1] 屠鹏飞[1,2] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]北京大学药学院天然药物与仿生药物国家重点实验室,北京100191

出　　处：《中国中药杂志》2009年第19期2481-2484,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学杰出青年基金项目(30525043)

摘　　要：目的：建立RRLC-UV法同时测定丹参药材中9种酚酸的含量。方法：以3，4-二羟基苯乙酸为内标，采用Agilent Zorbax Eclipse PlusC18（2．1mm×50mm，1．8μm）色谱柱，以乙腈-0．1％甲酸溶液为流动相梯度洗脱，流速0．3mL·min^-1，检测波长质量286nm。结果：这9种成分在测定质量浓度范围内与色谱峰面积线性关系良好，相关系数不低于0．9995。仪器精密度和方法精密度RSD均小于2．4％，9种成分平均回收率在96．7％～102．6％，RSD均小于3．1％（n=3）。结论：该方法简便、快速，结果准确，重复性好，适用于丹参药材的质量控制。Objective ： To establish a RRLC-UV method for simultaneous determination of nine phenolic acids in＇Salvia miltiorrhiza. Method： 3,4-Dihydroxyphenylacetic acid was used as internal standard, and analysis was carried out on an Agilent Zorbax Eclipse Plus C18 （2- 1 mm × 50 mm, 1.8μm） column with acetonitrile-0. 1% formic acid aqueous solution as mobile phases in gradient elution. The flow rate was 0. 3 mL · min^-1, and the UV detector was monitored at 286 nm. Result： All calibration curves showed good linear regression within test ranges （r≥0. 999 5 ） ; and the overall recoveries were in the range of 98.2%-102. 6% , with RSD less than 3. 1% （n = 3）. The overall RSD of precision test were less than 2.4%. Conclusion ： The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of S. miltiorrhiza.

关 键 词：丹参 RRLC—UV 酚酸 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]