滇白珠抗风湿性关节炎活性成分研究  

Effects of Gaultheria yunnanensis on adjuvant arthritis in rats

作　　者：熊玉兰[1] 肖冰[1] 马小军[2] 李长龄[1] 郑俊华[3] 叶加[1] 

机构地区：[1]北京大学药学院分子与细胞药理学系,北京100191 [2]中国医学科学院药用植物研究所,北京100193 [3]北京大学药学院天然药物学系,北京100191

出　　处：《中国中药杂志》2009年第19期2516-2519,共4页China Journal of Chinese Materia Medica

摘　　要：目的:对滇白珠的各提取液进行抗大鼠佐剂性关节炎活性筛选,为临床应用滇白珠治疗风湿性关节炎提供实验基础。方法:制备滇白珠的水提取总液、乙醇提取总液、醋酸乙酯提取液、正丁醇提取液、乙醇提取总液剩余部分液。滇白珠的正丁醇部分,在大孔树脂中用不同溶液洗脱,得到水洗脱部分、30%乙醇洗脱部分和95%乙醇洗脱部分。采用大鼠佐剂性关节炎模型,观察滇白珠不同部分提取液对大鼠关节肿胀的影响。结果:滇白珠的水提液组(A)、酸酸乙酯提取液组(C)和正丁醇提取液组(D)可明显减轻大鼠关节肿胀程度,正丁醇提取液组(D)作用最强(P<0.05,P<0.01)。进一步研究显示,滇白珠正丁醇提取液中水洗脱液和30%乙醇洗脱液均有抗大鼠佐剂性关节炎作用,而30%乙醇洗脱液作用更明显(P<0.05,P<0.01)。结论:滇白珠正丁醇提取液30%乙醇洗脱部分有明显的抗大鼠佐剂性关节炎作用。Objective： Gaultheria yunnanensis, are used widespreadly in the south of China to treat rheumatoid arthritis. The aim of this study was to provide an experimental basis for G. yunnanensis to therapy rheumatoid arthritis. Method ： We prepared water extracts, ethanol extracts, n-butanol extracts, ethyl acetate extracts and the rest of ethanol extracts from G. yunnanensis. Then, the nbutanol extracts were applied to macroporous resin and eluted with water, 30% ethanol, and 95% ethanol. Rheumatoid arthritis was induced by Freund＇s complete adjuvant injected into right postpedes in Wistar rats which was utilized to elucidate the anti-inflammatory effect of different extracted liquid of G. yunnanensis. Rats were intragastric injected （ig） with extracts as experimental group or normal saline as control group. Result： Freund＇s complete adjuvant induced arthritis was successfully established： paw edema were increased after Freund＇s complete adjuvant injection, peaked at 2 or 3 day, then decreased, the paw edema were increased again at 7 or 8 day, and persisted 15 d. Water extracts, n-butanol extracts or ethyl acetate extracts could a significantUy decrease the paw edema as compared with the control group （ P 〈 0.05, P 〈0.01 ）. The effect of n-butanol extracts was the most powerful. Further, n-butanol extracts eluant with water and 30% ethanol decreased the paw edema. The activity of extracts eluant with 30% ethanol was stronger than that of eluant with water. Conclusion： G. yunnanensis displays considerable effects against Freund＇s complete adjuvant induced arthritis in rats, which is in concordance with clinical practice, n-Butanol extracts and both of the eluants with water and 30% ethanol produce a significant decrease in the paw edema. 30% ethanol eluants show a stronger activity than others. The effects against rheumatoid arthritis of different parts of G. yunnanensis differ in degree. It is deserved to explore the potential mechanisms of anti-inflammtion of the G. yunnanensis, especiall

关 键 词：滇白珠 风湿性关节炎 佐剂性关节炎 

分 类 号：R285[医药卫生—中药学]