猪胰酶、胰岛素与胰蛋白酶抑制剂的联产  

Co-production of pancreatin,insulin and trypsin inhibitor

作　　者：李晓红[1] 邓如伟[1] 余蓉[1] 余珂[1] 王德明[2] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]西南财经大学校医院,四川成都610074

出　　处：《华西药学杂志》2009年第5期480-482,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的以猪胰浆为原料,联产胰酶、胰岛素(INS)与胰蛋白酶抑制剂(TI)。方法猪胰浆用中性醇处理,得到胰渣和上清液(I);胰渣经过聚乙二醇-6000沉淀得到胰酶;I用酸沉淀去掉杂质后再沉淀得到INS粗品和上清液(II);INS粗品经DE-AE-Sepharose FF层析纯化;II经Trypsin-壳聚糖多孔珠亲和吸附得到TI。结果胰酶的回收率为15.1%±0.6%,主要成分胰蛋白酶、胰脂肪酶和胰淀粉酶的含量分别为(5.13±0.17)×103、(38.47±1.16)×103、(58.27±2.49)×103U.g-1;INS的回收率为73.7±9.0 mg.kg-1(n=3),效价为27.25±0.34 U.mg-1,并具有完整的生物学活性;TI比活为102.59 U.mg-1,产率为33.86 U.g-1。结论胰酶、INS与TI的联产极大地提高了胰脏的利用率。OBJECTIVE To co - produce pancreatin, insulin （INS） and trypsin inhibitor （TI） from porcine pancreas homogenates. METHODS The pancreas homogenates was treated with neutral alcohol to obtain pancreas precipitate and superuatant （Ⅰ）. Pancreas precipitate was treated with polyethylene -6000 to obtain pancreatin, and after precipitated with acid to remove the impurities, Ⅰ was precipitated to obtain crude INS and supernatant （Ⅱ）. Crude INS was purified by DEAE - sepharose FF chromatography and Ⅱ was treated with trypsin - chitosan porous beads to obtain TI. RESULTS The weight recovery of pancreatin was 5. 13 ± 0. 17 ×10^3 , 38.47 ± 1.16 × 10^3 and 58.27 ± 2.49 × 10^3 U· g^- 1 , whose main components were 15.1% ± 0.6% （ n = 3 ）. The recovery of INS was 73.7 ± 9.0 mg· kg^ - 1 and the purified INS, with specific activity of 27.25 ± 0.34 U· g^ - 1 （ n = 3 ）, showed natural biological activity. The recovery of TI was 102.59 U· mg^-1, with specific activity of 33.86 U· g^ - 1 pancreas. CONCLUSION The co - production of pancreatin , INS and TI has enhanced the utilization of pancreas greatly.

关 键 词：胰酶 胰岛素 胰蛋白酶抑制剂 联产 猪胰脏 

分 类 号：R915[医药卫生—微生物与生化药学]