LC-MS分析艾塞那肽的肽图  

Peptide mapping analysis of Exenatide by LC-MS spectrometry

作　　者：付金香[1] 霍建丽[1] 田垒[1] 刘玉雪[1] 孔毅[1] 

机构地区：[1]中国药科大学生命科学与技术学院生物制药教研室,江苏南京210009

出　　处：《华西药学杂志》2009年第5期544-545,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的用液质联用技术(LC-MS)分析艾塞那肽的肽图。方法用胰蛋白酶切RP-HPLC肽图法测定艾塞那肽肽图,用ESI-LC/MS技术分析肽段的精确相对分子量。结果根据肽段的色谱保留时间、相对分子质量归属出肽图中各肽段所在的色谱峰。结论LC-MS是验证和分析蛋白质结构的高效准确方法。OBJECTIVE To analyze the peptide mapping of Exenatide by LC/MS. METHODS The peptide mapping was analyzed by trypsin digestion and RP - HPLC analysis. The relative molecular weight of the peptide fragments was measured by ESI - LC/MS. RESULTS The peptide fragments of Exenatide in the peptide mapping were assigned by analyzing the retain time and relative molecular weight. CONCLUSION The study proves that LC - MS is a good method to analyze peptide mapping of protein with the advantage of sensitivity, high speed and accuracy.

关 键 词：肽图谱分析 艾塞那肽 胰蛋白酶切RP-HPLC肽图法 液相色谱-质谱联用 

分 类 号：R917[医药卫生—药物分析学]