灰绿藜愈伤组织的诱导·继代及植株再生研究  

Study on the Callus Induction,Subculture and Plant Regeneration of Chenopodium glaucum Linn

作　　者：蒋刚强[1] 黄玲[1] 窦辉[1] 

机构地区：[1]新疆出入境检验检疫局技术中心,新疆乌鲁木齐830063

出　　处：《安徽农业科学》2009年第29期14184-14187,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]通过诱导愈伤组织分化,建立灰绿藜植株再生方法。[方法]以灰绿藜茎和叶为材料,研究不同种类、不同浓度的植物生长调节剂对灰绿藜愈伤组织诱导和继代、不定芽分化及再生植株生根与移栽的影响。[结果]NAA、2,4-D、IAA在单独使用时,一定浓度范围内均有愈伤组织产生,利用不同的外植体,最佳的诱导组合培养基分别是茎为MS+NAA 4.0mg/L+6-BA 0.5mg/L,叶为MS+2,4-D 4.0mg/L+6-BA 0.2mg/L,光照有利于愈伤组织的诱导。在优化灰绿藜愈伤组织继代培养条件时,发现5.0mg/L的抗坏血酸(VC)对于灰绿藜愈伤组织的褐变有良好的抑制作用,继代培养基中较好的组合为MS+2,4-D 0.5mg/L+6-BA 0.5mg/L。愈伤组织不定芽分化培养基为MS+6-BA 2.0mg/L+NAA 0.05mg/L,根分化的培养基为1/2 MS+NAA 0.2mg/L。[结论]该研究为灰绿藜组织培养中适宜的培养基选择提供了科学依据。[ Objective ] The research aimed to study callus induction of Chenopodium glaucum. [ Method ] Taking the stems and leaves of C. glaucum as materials, the effects of different kinds and concentrations of plant growth regulator on the callus induction, subculture, adventitious bud differentiation and the rooting and transplanting of regenerated plants of C. glaucum were studied. [ Result] The results showed that the best callus induction medium was MS medium with NAA 4.0 mg/L ＋ 6-BA 0.5 mg/L for the stem and MS with 2,4-D 4.0 mg/L ＋ 6-BA 0.2 mg/L for the leaf respectively. The calli were induced more efficiently by light treatment than dark. Then callus subculture was optimized, that is cultured with the addition of Vc 5.0 mg/L, 2,4-D 0.5 mg/L ＋ 6-BA 0.5 mg/L, and the callus could inhibit browning effectively with Vc after continuous subculture. The MS medium with 6-BA 2.0 mg/L ＋ NAA 0.05 mg/L was preferable for inducing adventitious shoot . And the 1/2 MS medium with NAA 0.2 mg/L was suitable for the root differentiation. [ Conclusion] This study will provide the scientific reference for choosing the feasible medium in tissue culture of Chenopodium glaucum.

关 键 词：灰绿藜 愈伤组织诱导 继代培养 植株再生 

分 类 号：S603.6[农业科学—园艺学]