耐亚胺培南铜绿假单胞菌对β-内酰胺类抗菌药物耐药相关基因的研究  

β-lactamases-resistant Genes in Imipenem-resistant Pseudomonas Aeruginosa

作　　者：李丰良[1] 高玉红[1] 侯香萍[1] 刘海云[1] 马美芬[1] 

机构地区：[1]云南省第一人民医院检验科,昆明市650032

出　　处：《中国药房》2009年第29期2268-2269,共2页China Pharmacy

摘　　要：目的:研究耐亚胺培南铜绿假单胞菌(IMPRPa)β-内酰胺类抗菌药物耐药相关基因的存在情况。方法:对60株临床分离的IMPRPa,采用聚合酶链(PCR)方法检测β-内酰胺类抗菌药物耐药相关基因IMP、VIM、OprD2,并对部分IMP、VIM基因的PCR扩增产物进行序列分析。结果:60株受试菌中,β-内酰胺类抗菌药物耐药相关基因检测阳性为:IMP7株,VIM18株,有3株菌同时携带IMP、VIM基因,IMP、VIM基因序列分析为IMP-1型和VIM-2型,OprD2基因缺失29株。结论:OprD2蛋白通道缺失和产金属β-内酰胺酶是IMPRPa耐亚胺培南的主要机制。OBJECTIVE： To screen and analyze the β- lactamases - resistant genes in imipenem - resistant pseudomonas aeruginosa （IMPRPa） . METHODS： β-lactamases- resistant genes including IMP, VIM and OprD2 in 60 clinically isolated IMPRPa strains were detected by polymerase chain reaction （PCR）, and sequence analysis was performed on the PCR amplified products of some of IMP and VIM genes. RESULTS： Of the 60 IMPRPa strains, β- lactamases- resistant positive genes were as follows： IMP gene was positive in 7 strains, VIM gene was positive in 18 strains, and both IMP and VIM genes were positive in 3 strains. Sequencing of the PCR products of IMP and VIM genes were IMP - 1 and VIM - 2 types, and OprD2 gene deletion was noted in 29 strains. CONCLUSION： OprD2 protein channel deletion and metal -producing β-lactamases are the chief mechanisms for the resistance of IMPRPa to imipenem.

关 键 词：铜绿假单胞菌 Β-内酰胺酶类 抗菌药物 耐药基因 

分 类 号：R969.3[医药卫生—药理学]