二氧化氯用于牙科水道消毒灭菌的效果评估  

Evaluation of A Chlorine Dioxide Disinfectant for Dental Unit Waterlines

作　　者：金爱琼[1] 赵俊颖[1] 宁克勤[1] 张磊[1] 

机构地区：[1]深圳市第二人民医院医保办,广东深圳518035

出　　处：《中华医院感染学杂志》2009年第20期2733-2735,共3页Chinese Journal of Nosocomiology

摘　　要：目的调查使用二氧化氯消毒剂抑制牙科水道内细菌生长、定植的效果。方法随机选择22个有独立供水系统的牙科水道单元,其中3个单元以及自来水作为对照;分别在水道处理前、处理后每周1次连续5周采集23个水道的样本;将这些水样本系列稀释、置于R2A琼脂培养板并设平行对照,于37℃孵育7 d,计数菌落。结果3个对照水道的菌落数中位值为8440 CFU/ml,而19个经消毒处理水道的菌落数中位值为9160 CFU/ml;1周后,19个处理水道中的18个菌落数<200 CFU/ml,到4周时,所有处理水道中的菌落数均为0 CFU/ml,并维持到第5周;扫描电镜检查显示类似结果,即经过化学消毒处理的牙科水道样本中几乎无生物膜,而未处理的对照水道有明显的生物膜形成。结论在牙科水道中使用二氧化氯消毒剂可以有效抑制细菌生长和定植,使牙科用水达到美国牙科协会(ADA)制定的<200 CFU/ml的目标。OBJECTIVE To investigate the use of a chlorine dioxide-based dental unit waterline （DUWL） treatment to reduce the colonization and growth of heterotrophic bacteria. METHODS Twenty-two dental units with selfcontained water systems were randomly selected. Three of the units and tap water served as controls. Twentythree water samples were taken at baseline and once a week for five weeks. They were serially diluted, spreadplated in duplicate onto R2A agar plates and incubated at 37 ℃ for 7 days. RESULTS At baseline, the 3 control DUWLs had a median count of 8440 colony-forming units/milliliter （CFU/ml） and the 19 treated DUWLs had a median count of 9160 CFU/ml. By week 1, 18 of the 19 treated DUWLs had counts of less than 200 CFU/ml, and by week 4, the median count for all of the treated DUWLs was 0 CFU/ml. The measurement at week 5 showed that the reduction to below 200 CFU/ml had been maintained. Scanning electron examination revealed a similar results, with biofilm accumulation more evident in the untreated control specimens. CONCLUSIONS Using a chlorine dioxide-based disinfectant in DUWLs can achieve the American Dental Association （ADA） goal of less than 200 CFU/ml of heterotrophic bacteria per milliliter of unfiltered output water.

关 键 词：牙科水道 二氧化氯 化学消毒剂 菌落计数 

分 类 号：R187.1[医药卫生—流行病学]