糖蛋白/糖肽的分离富集方法  

Separation and Enrichment of Glycoproteins/Glycopeptides

作　　者：曹晶[1,2] 聂爱英[2] 陈瑶函[2] 王胜[1] 陆豪杰[1,2] 杨芃原[1,2] 

机构地区：[1]复旦大学化学系,上海200433 [2]复旦大学生物医学研究院,上海200032

出　　处：《化学进展》2009年第9期1888-1894,共7页Progress in Chemistry

基　　金：国家自然科学基金项目(No.20735005)资助

摘　　要：蛋白质糖基化是一种重要的翻译后修饰,糖基化对蛋白质的结构和功能有着重要的影响。目前,作为蛋白质组学的一个组成部分,糖蛋白质组学是备受关注的研究热点,而从复杂的生物样品体系中富集糖蛋白/糖肽是蛋白质糖基化研究的重点和难点。本文就糖蛋白/糖肽分离富集方法的研究进展和应用作了简要概述。这些方法包括常用的凝集素亲和法、硼酸法、肼化学法和亲水作用法,还包括分子筛法、强阳离子交换法等新方法。Protein glycosylation as an important post-translational modification has a significant effect on the structures and functions of proteins. As a section of proteomics, glycoproteomics is in the spotlight currently, and the efficient separation and enrichment of glycoproteins/glycopeptides from complex biological samples is the key and difficult point of glycoproteome research. In this paper, the progress in methods for the separation and enrichment of glycoproteins/glycopeptides and their applications are overviewed. These methodologies not only include most-often-used methods such as lectin-based affinity chromatography, boronic acid method, hydrazide chemistry and hydrophilic chromatography, but also some novel methods, for example, size exclusion chromatography and strong cation exchange enrichment.

关 键 词：糖蛋白质组学 糖蛋白 糖肽 富集 

分 类 号：O652.6[理学—分析化学] O629.7[理学—化学]