石墨炉原子吸收光谱法测定血清中非蛋白结合铜和锌  

Determination of Trace Free Copper and Zinc in Serum Samples by Graphite Furnace Atomic Absorption Spectrometry after Two-Step Precipitation of Protein

作　　者：张源[1] 林哲绚[1] 李伟秋[1] 张俏忻[1] 李慧[1] 罗红军[1] 罗文鸿[1] 

机构地区：[1]汕头大学医学院中心实验室,广东汕头515041

出　　处：《光谱学与光谱分析》2009年第10期2864-2866,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30271139);广东省自然科学基金项目(022124)资助

摘　　要：建立两步沉淀蛋白质-石墨炉原子吸收法测定血清中非蛋白结合铜和锌的方法。无水乙醇以2∶1与血清混合后,再经70℃温度变性处理,可将蛋白质完全沉降。用氘灯校正背景,石墨炉原子吸收法进行测定,方法检测限:Cu,1.2μg.L-1,Zn,0.098μg.L-1。回收率:Cu,92.3%～104%,Zn,为90%～107%。利用此方法测定了肿瘤患者血清中游离铜与锌,并与健康人作了对比。A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1 ： 2. The mixture was subsequently denatured at 70 ℃ and centrifuged to precipitate proteins. The determination, limit of copper （3σ） was 1.2μg ·L^-1 and the recovery was 92. 3%- 104%, and the determination. Limit of zinc（3σ） was 0. 098μg ·L^-1 and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people.

关 键 词：铜 锌 GFAAS 蛋白质沉淀 

分 类 号：O657.3[理学—分析化学]