马来西亚食蟹猴B病毒相关抗体检查  

Detection of Antibodies Against B Virus in Malaysia Crab-Eating Macaques(Macaca fascicularis)

作　　者：刘欢[1] 张伟[1,2] 李晓平[1] 王起 白素英[1,2] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]国家林业局野生动植物检测中心,哈尔滨150040 [3]吉林省磐石市公安局,吉林132300

出　　处：《实验动物科学》2009年第4期60-62,共3页Laboratory Animal Science

摘　　要：目的提供马来西亚食蟹猴B病毒相关抗体阳性率,为我国从马来西亚进行食蟹猴引种提供参考依据。方法对野外捕捉的1 916只食蟹猴后股静脉采血,低温静置,高速离心、分离血清,用玻片免疫酶法（EIA）和ELISA诊断试剂盒法两种方法对血清进行检测。结果总的B病毒相关抗体阳性率为68.5%,其中年龄〈2岁、2-4岁、4-6岁的B病毒相关抗体阳性率分别为53.0%6、3.8%和76.4%。结论马来西亚食蟹猴B病毒相关抗体阳性率较大;B病毒相关抗体的阳性率是随着年龄的增加而增加;B病毒的感染不受性别的影响。因此在引种过程中应选用年龄较小者。Objective To investigate the positive rate of antibodies against B virus in Malaysia Crab-eating Macaques （Macaca fascicularis）. Methods Blood were collected from the femoral veins of total 1916 Macacafascicularis captured in the wild, then sera were isolated and examined using the immune enzymatic slide （EIA） and ELISA diagnostic kit Results The total positive rate of antibodies against the B virus was 68.5 %. For the monkeys below 2 years, 2 to 4 years and 4 to 6 years old, positive rate was 53.0% , 63.8% and 76.4% respectively. Conclusion The positive rate of antibodies against B virus is high and increases with aging in Macaca fascicularis. Infection of B virus is not influenced by gender.

关 键 词：B病毒 食蟹猴 马来西亚 

分 类 号：S865.1[农业科学—野生动物驯养]