经冠状动脉移植白体BM—MNCs治疗猪急性心肌梗死的作用  

Experimental study of autologous BM-MNCs by intracoronary transplantation into ischemic myocardium of AMI in swines

作　　者：孙林[1] 张戈[1] 光雪峰 左明鲜 周旭[1] 章体玲 李劲松[3] 白文伟[1] 楚天舒[1] 黄桂琴[4] 陆地[4] 杨达宽[1] 

机构地区：[1]昆明医学院第二附属医院心血管内科,云南省冠心病基础研究室650101,云南省 [2]昆明市延安医院心血管内科 [3]云南省第二人民医院心脏中心外科 [4]昆明医学院生物医学工程中心

出　　处：《中国心血管病研究》2009年第3期207-209,共3页Chinese Journal of Cardiovascular Research

摘　　要：目的探讨冠状动脉内移植自体骨髓单个核细胞（BM—MNCs）治疗猪急性心肌梗死的有效性。方法实验动物分为冠脉内移植MNCs组（n=6）及冠脉对照组（n=5）。冠脉内移植BM—MNCs后4周观察移植细胞归巢情况、小血管密度、心功能变化。结果冠脉内MNCs移植后4周，心肌细胞间可见发蓝色荧光的移植细胞，散在分布，心肌细胞间有较多新生毛细血管并可见新生的心肌样细胞。移植组小血管密度、左室射血分数（LVEF）及短轴缩短率（FS）明显高于对照组（P〈0．01），左室舒张末期内径（LVEDd）低于对照组（P〈0．05）。结论经冠脉移植的BM—MNCs可归巢到宿主心肌，有促进缺血心肌血管新生、改善左室收缩功能、减轻心室重构的作用。Objective To test the effectiveness of BM-MNCs by intracoronary transplantation. Methods 11 swines were divided into two groups： BM-MNCs group （n=6） and confrol group （n=5）. Four weeks after implantation, BM-MNCs homing, blood vessels density, heart function were observed. Results Four weeks after implantation, blue fluorescence cells can be detected in host myocardium. Many newborn capillary vessels and a few cardiomyocytes-like were observed. Blood vessels density and LVEF and FS in BM-MNCs group were significantly higher than those in control group （P〈0.01） and LVEDd were lower than those in control group （P〈0.05）. Conclusion Intracoronary transplantation BM-MNCs are capable of homing to host myocardium, which can induce angiogenesis and improve cardiac function and attenuate remodeling of the left ventricle.

关 键 词：骨髓单个核细胞 移植 心肌梗死 猪 

分 类 号：Q95-33[生物学—动物学] R542.2[医药卫生—心血管疾病]