海水网箱区沉积物中硫酸盐还原菌(SRB)的分离纯化和鉴定  

The Isolation and Identification of SRB Strains in the sediment of marine aquaculture cage area

作　　者：胡德蓉[1,2] 林钦[2] 柯常亮[2] 

机构地区：[1]上海水产大学,200090 [2]农业部渔业生态环境重点开放实验室中国水产科学研究院南海水产研究所,广州510300

出　　处：《水产科技》2009年第2期9-13,共5页

基　　金：科技部社会公益研究专项基金项目(2000DIB50175)

摘　　要：目前网箱养殖水域的硫酸盐还原菌(SRB)的危害表现日益严重,海水网箱沉积物中SRB分离鉴定尚属空白,针对不同属种的SRB开展生物修复具有一定的意义。在大亚湾大鹏澳网箱养殖沉积物中分离出25株硫酸盐还原菌纯菌株,通过对菌株的形态观察,生理生化特征和遗传特征的一系列研究,结果表明所有菌株均含有脱硫弧菌素,被鉴定为脱硫弧菌属(Desulfovibrio)其中CSRB2、CSRB4、ESRB2鉴定为脱硫脱硫弧菌(Desulfovibrio Desulfovibricans),ASRB4、BSRB1菌株鉴定为脱硫脱硫弧菌河口亚种(Desulfovibrio Desulfovibricans subsp.aestuarii),其余菌株鉴定为需盐脱硫弧菌种(Desulfovibrio salexigens)。此海区SRB种类数量特征是需盐脱硫弧菌>脱硫脱硫弧菌>脱硫脱硫弧菌河口亚种。There was seriously harmed by the sulfate reducing bacteria （SRB） in the marine aquaculture cage area,the SRB＇s isolation and identification was still blank in the marine aquaculture area, there was an important significance in the research of Bio-remediation for different SRB species in the marine aquaculture area. There are 25 bacteria strains were isolated from the sediment of Dapeng＇ao Cove marine aquaculture cage area. Applying morphological, physiological, biochemical and genetic characters analysed the isolated bacterias. The results show All of them are classified into Desulfovibrio. CSRB2,CSRB4,ESRB2 are identified with Desulfovibrio Desulfovibricans, ASRB4,BSRB1 are Desulfovibrlo Desulfovibricans subsp.aestuarii, the others are identified with Desulfovibrio salexigens, the quantitative character of SRB in this area is Desulfovibrio salexigens〉 Desulfovibrio Desulfovibricans〉 Desulfovibrio Desulfovibricans subsp.aestuarii.

关 键 词：海水网箱沉积物 硫酸盐还原菌 分离纯化 鉴定 

分 类 号：S858.31[农业科学—临床兽医学] TG172.7[农业科学—兽医学]