纳米羟基磷灰石人工心脏机械瓣膜对细胞毒性评价  

The evaluation cytotoxicity of mechanical heart valve of hydroxyapatite （HA） film complex materials and human blood vessel endothelial cells

作　　者：程光存 严中亚 沙自明[2] 罗乐[3] 方晓东[4] 陶汝华[4] 

机构地区：[1]安徽医科大学附属省立医院胸心外科,合肥市230001 [2]巢湖市人民医院胸心外科 [3]合肥工业大学理学院 [4]中国科学院安徽光学精密机械研究所

出　　处：《中国心血管病研究》2009年第6期447-449,共3页Chinese Journal of Cardiovascular Research

基　　金：安徽省人才培养及科研带头人专项基金（2005）58号

摘　　要：目的用四甲基偶氮唑蓝（MTT）法检测羟基磷灰石（HA）对人脐带内皮细胞可能存在的细胞毒性。方法用MTT比色方法评价材料的细胞毒性。设常温漫提液组和高温浸提液组。两组均设置各自对应的阳性和阴性对照组。将加入浸提液和材料并培养72h后的各组样品分别在倒置相差显微镜下观察细胞在浸提液中及在材料边缘的形态变化及生长状况，以细胞毒性实验来评价生物材料生物相容性。参照国际标准化组织（ISO）制定的细胞毒性实验的标准，即ISO10993—5：1992（医疗器械生物学评价细胞毒性实验体外法》之评价标准和要求。结果材料浸提液与细胞共培养组24h时观察常温浸提液、高温浸提液及阴性对照组的HUVEC细胞形态、数量，各实验组与阴性对照组差异无统计学意义。结论HA材料对人脐带内皮细胞生长抑制反应级别为1级或0级，无明显毒性作用，有良好的细胞相容性。Objective In order to detect cytotoxicity of the hydroxyapatite （HA）that probably exists to human umbilical cord vein endothelial celI（HUVEC） using MTT method. Methods We used MTT to evaluate the material cytotoxicity of HA. The normal and the heat temperature leaching liquor class were set up and they all had control group respectively. HUVEC in leaching liquor observed respectively under the inverted phase contrast microscope after the 72 h and in leaching liquor and at the material edge, HUVEC appearance and growth status were observed to evaluate the biocompatihility of the material. According to the standard that made by the Interna- tional Organization for Standardization（ISO）, ISO10993-5：1992 《cytotoxicity of the biology evaluation of the medical treatment apparatuses experiment method in vitro》,we evaluated cytotoxicity. Results HUVEC was cultivanted in leaching liquor of HA and HUVEC Appearance, amount were observed after 24 h. There were no obvious difference between experimental groups and negative control group. Conclusion HA displayed no obvious cytotoxicity and has good biecompatibility.

关 键 词：羟基磷灰石 人脐带静脉内皮细胞 四甲基偶氮唑蓝 细胞毒性 

分 类 号：R318.2[医药卫生—生物医学工程] R654.2[医药卫生—基础医学]