羟基磷灰石膜复合材料上人血管内皮细胞分泌功能的研究  

Study on biocompatibility of hydroxyapatite （ HA ） film complex materials and human blood vessel endothelial cells

作　　者：程光存 严中亚 沙自明[2] 罗乐[3] 方晓东[4] 陶汝华[4] 

机构地区：[1]安徽医科大学附属省立医院胸心外科,合肥市230001 [2]安徽巢湖市人民医院胸心外科 [3]合肥工业大学理学院 [4]中国科学院安徽光学精密机械研究所

出　　处：《中国心血管病研究》2009年第6期450-453,共4页Chinese Journal of Cardiovascular Research

基　　金：安徽省人才培养及科研带头人专项基金（2005）58号

摘　　要：目的探讨羟基磷灰石膜复合材料上人血管内皮细胞分泌功能的变化。方法将人血管内皮细胞与纳米相羟基磷灰石（HA）薄膜复合材料体外复合培养，进行形态学和功能测定，同时分别测定HA材料组和空白对照组中血管内皮细胞分泌一氧化氮（NO）、前列环素（PGI2）水平。结果人血管内皮细胞能在纳米相羟基磷灰石薄膜复合材料上良好地黏附、增殖、生长。人脐静脉血管内皮细胞分泌NO和PGI2在HA材料组和空白对照组差异无统计学意义（P〉0．05）。结论相羟基磷灰石膜复合材料上人血管内皮细胞分泌功能正常，说明纳米相羟基磷灰石薄膜复合材料具有良好的细胞相容性，可作为组织工程化机械瓣膜材料。Objective To study the secretion function change of human vascular endothelial cells in nanometerie hydroxyapatite （HA） film complex materials. Methods Human vascular endothelial cells and nanometefic HA film complex materials were co-cultivated in vitro. Morphological and functional indexes of human vascular endothelial cells were measured. The levels of nitric oxide （NO） and PGI2 secreted by vascular endothelial ceils of HA group and normal control group were also measured. Results Human vascular endothelial cells eould well adhere to nanometeric HA film complex materials and could well multiply and grow. The level of NO and PGI2 secreted by human vascular endothelial cells had no significant difference （P〉0.05）. Conclusion The secretion function of human vascular endothelial cells in nanometerie hydroxyapatite （HA）film are Normal. Material has good biocompatibility and could be used as tissue engineering artificial mechanical valve material.

关 键 词：脉冲激光沉积 羟基磷灰石薄膜 组织工程 人血管内皮细胞 细胞培养 血管内皮细胞功能 

分 类 号：R318.2[医药卫生—生物医学工程] R654.2[医药卫生—基础医学]