椰甲截脉姬小蜂寄生对椰心叶甲幼虫体内几种酶活性的影响  

Effect of Parasitism by Asecodes hispinarum on the Activity of some Enzymes in larvae of Brontispa longissima

作　　者：宋晓君[1,2,3] 陈青[1] 覃伟权[2] 唐超[1] 金启安[1] 温海波[1] 彭正强[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737 [2]中国热带农业科学院椰子所,海南文昌571339 [3]海南大学环境与植物保护学院,海南儋州571737

出　　处：《热带作物学报》2009年第5期699-703,共5页Chinese Journal of Tropical Crops

基　　金：973项目(No.2009CB119200);国家科技支撑计划项目(No.2006BAD08A18);科技部公益研究专项(No.2005DIB4J043);海南省科学技术厅重点项目(No.080148);国家科技支撑计划项目(No.2007BAD48B06;2007BAD48B01);中央级公益性科研院所基本科研业务费(No.2008hzs1J010)资助

摘　　要：采用分光光度法测定被椰甲截脉姬小蜂寄生0.5～4d内的椰心叶甲3龄和4龄幼虫体内几种酶活性的变化。结果表明:寄生后,寄主幼虫体内过氧化氢酶(CAT)和过氧化物酶(POD)活性均高于对照;多酚氧化酶(PPO)活性受抑制;酯酶(EST)活性先升高后降低。结果揭示了椰心叶甲幼虫对寄生产生的生理防御反应。By using spectrophotometric method, the activities of several enzymes, including catalase （CAT）, peroxidase （POD）, polyphenol oxidase （PPO） and esterase （EST）, were determined in Brontispa longissima larvae parasitized by Asecodes hispinarum. The results showed that the activities of CAT and POD were higher in the parasitized larvae than in the unparasitized larvae at the same stage, that the activity of PPO was inhibited in parasitized larvae, and that the activity of EST was stimulated initially and then inhibited in the parasitized larvae. The changes of the enzymatic activities were helpful to the successful development of A.hispinarum in the host larvae. This study indicated the B.longissima larvae had a physiological defensive action against A. hispinarum, which can be used to probe into the relationship between B. longissima and A. hispinarum.

关 键 词：椰心叶甲 椰甲截脉姬小蜂 酶活性 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]