白菜黑斑病菌拮抗细菌LBC-1菌株的分离与鉴定  

Separation and Identification of Bacillus LBC-1 against Alternaria brassicae

作　　者：李春辉[1] 李术娜[2] 王纳贤[1] 杜淑涛[2] 王世英[2] 朱宝成[1,2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《中国蔬菜》2009年第20期29-34,共6页China Vegetables

摘　　要：从湖南、湖北、河北等地区菜地采集土样,采用抑菌圈法筛选对白菜黑斑病病原菌芸薹链格孢菌(Alternaria brassicae)具有拮抗作用的芽孢杆菌菌株。通过初筛得到具有拮抗作用的菌株20株,复筛出1株拮抗活性较高的菌株LBC-1。通过生理生化试验、形态特征观察及16S rDNA序列分析对其进行了种属鉴定。鉴定结果为菌株LBC-1属于解淀粉芽孢杆菌(Bacillus amyloliquefaciens),是1株广谱拮抗芽孢细菌菌株。Twenty strains of Bacillus were isolated by sampling soil from fields of various regions via preliminary screening. They have antagonistic activities against Alternaria brassicae. A strain named LBC - 1 with a relatively higher antagonistic activity was obtained via secondary screening. Strain LBC - 1 was characterized by physiological and biochemical experiments, morphological features observation, and 16S rDNA sequence analysis. Based on the results, strain LBC - 1 was finally identified as Bacillus amyloliquefaciens. Strain LBC -1 was a broad-spectrum antagonistic strains of spore-forming bacteria.

关 键 词：拮抗细菌 筛选 鉴定 解淀粉芽孢杆菌 

分 类 号：S436.34[农业科学—农业昆虫与害虫防治]