PCR在血液病原菌16S rRNA基因检测的诊断价值  

Diagnostic Value of Detection of 16S rRNA Gene of Pathogens from Blood by PCR

作　　者：喻长法[1] 叶丽君[1] 任应鹏[1] 段达荣[1] 阮荣华[1] 张仙森[1] 

机构地区：[1]台州市第一人民医院检验科,浙江台州318020

出　　处：《中华医院感染学杂志》2009年第19期2521-2523,共3页Chinese Journal of Nosocomiology

摘　　要：目的为早期诊断败血症,探索一种能快速检测病原菌感染的新方法。方法用PCR技术扩增实验室保留株10株的16S rRNA基因,以人类基因组DNA、HBV-DNA和白色假丝酵母菌为对照,检测该方法的特异性;采用10倍比稀释法进行该方法的灵敏度检测。结果对所测病原菌株均获得371 bp扩增产物,而与人基因组DNA、HBV-DNA和白色假丝酵母菌无交叉反应;PCR最低能检测1.5×104/L大肠埃希菌。结论16S rRNA基因PCR检测方法具有快速,特异性和敏感性高等特点。OBJECTIVE To explore study a method for rapid detection of bacterial infection in clinic to diagnose septicemia early. METHODS 16S rRNA gene of ten bacterial species was amplified with PCR, by using human genome DNA, HBV-DNA and Candida albicans as comparison. The sensitivity test was done by the method of gradual dilution of Escherichia coll. RESULTS The bacterial species were amplified and the products were 371 bp, but human genome DNA, HBV-DNA and C. albicans showed no amplification products. Sensitivity test showed that it could detect as low as 1.5×10^4/L of E. coll. CONCLUSIONS The method is rapid and highly specific and sensitive in detecting the existence of bacterial 16S rRNA gene.

关 键 词：16S核糖体核糖核酸 聚合酶链反应 病原菌 

分 类 号：R378[医药卫生—病原生物学]