血红素氧合酶-1及一氧化碳-胆红素系统对心力衰竭大鼠心功能的保护作用  

The protective effect of heme oxygenase-1/CO-bilirubin system on cardiac function in rat model of heart failure

作　　者：司晓云[1] 程友琴[1] 李小鹰[1] 韩丽娜[1] 谭国娟[1] 吴伦宽[2] 

机构地区：[1]解放军总医院,北京100853 [2]贵阳医学院

出　　处：《中华老年心脑血管病杂志》2009年第10期791-794,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的探讨经胃肠道给予氯化高铁血红素后.体内血红素氧合酶-1(HO-1)及一氧化碳-胆红素(CO-胆红素)的反应和对大鼠慢性压力负荷性心力衰竭(心衰)进程的影响。方法将成年雄性SD大鼠63只,随机分为血红素组、心衰组和对照组,每组21只。术后3周血红素组以60 mg·kg^(-1)·d^(-1)氯化高铁血红素灌胃,另外2组同时间灌以同体积生理盐水。并分别在4、8、12周检测血清HO-1、碳氧血红蛋白(COHb);测定尾动脉压;经颈动脉插管检测平均动脉压、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)以及左心室压力最大上升速率(+dp/dt_(max))和左心室压力最大下降速率(—dp/dt_(max))。结果与对照组比较,心衰组8、12周时HO-1和COHb含量明显升高(P<0.01);与心衰组比较,血红素组在4、8、12周时HO 1和COHb含量明显升高(P<0.01),8周时尾动脉压、平均动脉压和LVSP明显降低(P<0.01),8、1 2周时LVEDP明显降低(P<0.05,P<0.01),12周时±dp/dt_(max)明显升高(P<0.01)。结论经胃肠道给予氯化高铁血红素可对体内HO-1产生诱导作用;HO-1及CO-胆红素系统的诱导能减缓压力负荷性心衰大鼠心衰的进展,对心脏的收缩和舒张功能有保护作用。Objective To investigate the protective effect of herne oxygenase（HO）-1/CO-bilirubin system induced by hemin on cardiac function in the rat model of pressure-overload heart failure. Methods Sixty-three male SD rats were divided into control group（C group, n= 21） ,heart fail- ure group（HF group, n= 21） and heine group（Hm group, n= 21） randomly. Three weeks after establishing heart failure,the Hrn group began to receive daily intragastric administration of hemin（60 mg/kg） ,while the other groups were administered with equal volume of saline. The effect of hemin on the blood level of HO-1, carbon monoxide hemoglobin（COHb）.caudal arterial pressure（BP）, hemodynamic parameters including mean arterial pressure（MAP）, left ventricular sys tolic pressure（LVSP）,left ventricular end diastolic pressure（LVEDP） and ＋dp/dtmax were measured. Results Compared with other 2 groups, the blood levels of HO-1 and COHb in Hm group were obviously increased. The values of BP,MAP, LVSP in Hm group were decreased significantly at the end of 8 weeks and the LVEDP was significantly decreased at the end of 8 weeks and 12 weeks after treatment compared with HF group. Compared with HF group, the Tdp/dtmax in Hm group was obviously increased at the end of 12 weeks after treament. Conclusion HO-1 CO-bilirubin system can be induced by hemin through intragastic administration. The cardiac funcuon can be improved and heart failure can be slowed by induced HO-1/CO-bilirubin system m the rat model of pressure-overload heart failure.

关 键 词：血红素加氧酶-1 一氧化碳 胆红素 心力衰竭 氯化血红素 

分 类 号：R541.6[医药卫生—心血管疾病]